ABN1698

Anti-Aspa/Nur7 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Aspartoacylase, Aminoacylase-2, ACY-2, Aspa/Nur7, Anti-Aspartoacylase
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human, mouse, rat
• técnicas: immunohistochemistry: suitable; western blot: suitable
• Nº de acceso NCBI: NP_075602
• Nº de acceso UniProt: Q8R3P0
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ASPA(443)

Descripción

Descripción general

Aspartoacylase (EC 3.5.1.15; UniProt Q8R3P0; also known as Acy-2, Aminoacylase-2, Nur-7) is encoded by the Aspa (also known as Acy2) gene (Gene ID 11484) in murine species. Aspartoacylase catalyzes the deacetylation of N-acetylaspartate (NAA) to generate free acetate in the central nervous system (CNS). Mutations in the ASPA gene cause the autosomal recessive neurodegenerative Canavan disease (CD) that results in inadequate lipid/myelin synthesis during development. Immunohistochemical staining reveals that aspartoacylase colocalizes with the oligodendrocyte marker CC1 throughout the brain, indicating its role in myelination.

Inmunógeno

N-terminally his-tagged full-length recombinant mouse Aspa/Nur7.

Aplicación

Immunohistochemistry Analysis: A representative lot detected Aspa/Nur7-positive oligodendrocytes in corpus callosum/external capsule using free-floating mouse brain sections (Courtesy of Dr. John R. Moffett, Uniformed Services University of the Health Sciences).
Western Blotting Analysis: A representative lot detected Aspa/Nur7 in the cytosol, but not myelin, fraction from rat brain tissue homogenate (Madhavarao, C.N., et al. (2004). J Comp Neurol. 472(3):318-329).
Immunohistochemistry Analysis: A representative lot detected Aspa/Nur7 expression pattern in various regions of rat forebrain, including corpus callosum, cerebral cortex, hippocampal commissure (hc), fimbria, and anterior commissure (Madhavarao, C.N., et al. (2004). J Comp Neurol. 472(3):318-329).
Immunohistochemistry Analysis: A representative lot detected similar Aspa/Nur7 expression pattern as CC1 in various regions of rat forebrain, including the Purkinjie & axonal fiber layers of cerebellum, corpus callosum, as well as layer 2 of primary somatosensory cortex (Madhavarao, C.N., et al. (2004). J Comp Neurol. 472(3):318-329).

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This Anti-Aspa/Nur7 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of Aspa/Nur7.

Calidad

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Aspa/Nur7 in 10 µg of rat brain tissue lysate.

Descripción de destino

~37 kDa observed

Forma física

Protein G Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable