05-665-AF647

Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor^™ 647

clone 8E7, from mouse, ALEXA FLUOR^™ 647

• Sinónimos: Catenin beta-1, Beta-catenin, Active-β-Catenin
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• conjugado: ALEXA FLUOR^™ 647
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 8E7, monoclonal
• reactividad de especies: human, mouse
• reactividad de especies (predicha por homología): zebrafish (based on 100% sequence homology), rat (based on 100% sequence homology)
• técnicas: flow cytometry: suitable; immunocytochemistry: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001895
• Nº de acceso UniProt: P35222
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CTNNB1(1499) ; mouse ... Ctnnb1(12387) ; rat ... Ctnnb1(84353) ; zebrafish ... Ctnnb1(30265)

Descripción

Descripción general

Catenin beta-1 (UniProt P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, MRD19) gene (ORF OK/SW-cl.35, PRO2286; Gene ID 1499) in human. beta-Catenin is a multifunctional protein involved in the regulation of cell–cell adhesion and in the control of the Wnt signaling pathway. beta-Catenin contains in its central region 12 armadillo repeats that mediate its interaction with a variety of proteins including axin, adenomatous polyposis coli (APC), and T cell factor (TCF) family transcription factors. The C-terminal domain (CTD) of beta-catenin is implicated in transcriptional functions, while the N-terminal domain (NTD) contains several posttranslational modification (ubiquitination, acetylation, and phosphorylation) sites, including the Ser33/Ser37/Thr41 residues, important for beta-catenin stability regulation. In the absence of Wnt pathway activation, beta-catenin is retained in the cytoplasm in a “destruction complex” with Axin, APC, and GSK3beta, where it is constitutively destined for proteasomal degradation as a result of constant phosphorylation by GSK3beta. Activation of the Wnt pathway results in stabilization and nuclear translocation of beta-catenin, resulting in beta-catenin target genes transcription. In addition to GSK3beta, PKCalpha is also reported to phosphorylate the Ser33/37/Thr41 residues of beta-catenin, and beta-catenin Ser45 is a known phosphorylation site for CK1alpha. In addition, HDAC6 and p300/CBP-associated factor (PCAF) regulate beta-catenin Lys49 acetylation.

Especificidad

Recognizes active form of β-catenin, dephosphorylated on Ser37 or Thr41.

The antibody eptiope is 100% conserved in zebrafish.

Inmunógeno

Epitope: The epitope corresponds to amino acid residues 36-44 (HSGATTTAP) of human β-catenin.

Fusion protein corresponding to residues 1-100 of human β-catenin with a c-terminal histidine tag.

Aplicación

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Active-β-Catenin in NIH/3T3 cells.
Flow Cytometry Analysis: 0.1 µg of this antibody from a representative lot detected Active-β-Catenin in 1X10E6 A431 and HeLa cells.
The unconjugated version (Cat. No. 05-665) has been shown to work in WB, FC, ICC, IHC, IH(P).

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-Active-β-Catenin Antibody, clone 8E7, Alexa Fluor^™ 647 is validated for use in Immunocytochemistry, Flow Cytometry for the detection of Active-β-Catenin.

Calidad

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Active-β-Catenin in HeLa cells.

Descripción de destino

~85 kDa observed

Forma física

Protein G Purified

Purified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1 % sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Información legal

ALEXA FLUOR is a trademark of Life Technologies

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable