R1003

Ribonuklease T₁ aus Aspergillus oryzae

ammonium sulfate suspension, 300,000-600,000 units/mg protein

• Synonym(e): Guanyloribonuclease, Ribonucleate 3′-guanylo-oligonucleotidohydrolase
• CAS-Nummer: 9026-12-4
• EC-Nummer: 3.1.27.3 (BRENDA, IUBMB)
• EG-Nummer: 232-809-1
• MDL-Nummer: MFCD00132191
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Aspergillus sp. (Aspergillus oryzae)
• Qualitätsniveau: 200
• Form: ammonium sulfate suspension
• Spezifische Aktivität: 300,000-600,000 units/mg protein
• Mol-Gew.: 11068 by amino acid sequence
• Methode(n): cell based assay: suitable
• Eignung: suitable for separating native or denatured proteins, or nucleic acids
• Anwendung(en): cell analysis
• Lagertemp.: 2-8°C

Beschreibung

Anwendung

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .

Biochem./physiol. Wirkung

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3′-phosphate group of a guanidine ribonucleotide and 5′-hydroxyl of the adjacent nucleotide. The initial product is a 2′:3′ cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3′-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3′-GMP and oligonucleotides with a 3′-GMP terminal group.

Einheitendefinition

1 U produziert säure-lösliche Oligonukleotide äquivalent zu einer ΔA₂₆₀ von 1.0 in 15 Minuten bei pH 7.5 und 37°C, in einem Reaktionsvolumen von 1.0 mL. Substrat: Hefe RNA.

Physikalische Form

Suspension in 3.2 M (NH₄)₂SO₄-Lösung, pH ~ 6

Hinweis zur Analyse

Proteingehalt mittels E1%/280

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves