P3738

Protein Kinase G Iβ human

≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution

• Synonym(e): cyclic-Guanosine Monophosphate-dependent Protein Kinase 1β human
• EC-Nummer: 2.7.11.12 (BRENDA, IUBMB)
• MDL-Nummer: MFCD03458230
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Rekombinant: expressed in baculovirus infected Sf9 cells
• Qualitätsniveau: 200
• Assay: ≥95% (SDS-PAGE)
• Form: buffered aqueous glycerol solution
• Spezifische Aktivität: ≥1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))
• Mol-Gew.: 76 kDa (monomer)
• UniProt-Hinterlegungsnummer: Q13976
• Relevante Krankheit(en): cancer
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... PRKG1(5592)

Beschreibung

Anwendung

Protein Kinase G is a serine/threonine-specific protein kinase that is activated by cGMP. Protein Kinase G Iβ is used to induce apoptosis and inhibit cell proliferation.

Biochem./physiol. Wirkung

Protein Kinase G Iβ induces apoptosis in certain cell lines such as human breast cancer cell lines MCF-7 and MDA-MB-468. It inhibits cell proliferation and induces apoptosis in colon cancer cell lines.

Einheitendefinition

One unit will phosphorylate 1 micromole of VASPtide(RRKVSKQE) substrate per minute in 10 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM DTE and 0.2 mM EDTA.

Physikalische Form

Solution in 20 mM Tris buffer, pH 7.4, 1 mM EDTA, 1 mM β-mercaptoethanol, 100 mM NaCl, 10 U/ml Trasylol, and 50% glycerol.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable