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Merck

C0150

Sigma-Aldrich

Cathepsin B from human placenta

lyophilized powder, ≥5 units/mg protein

Synonym(e):

Cathepsin B1

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About This Item

CAS-Nummer:
EC-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54

Form

lyophilized powder

Qualitätsniveau

Spezifische Aktivität

≥5 units/mg protein

Mol-Gew.

24.5 kDa

Zusammensetzung

Protein, ~50% Lowry

Löslichkeit

H2O: soluble 1.0 mg/mL, clear

UniProt-Hinterlegungsnummer

Lagertemp.

−20°C

Angaben zum Gen

human ... CTSB(1508)

Allgemeine Beschreibung

Cathepsin B is encoded by the gene CTSB and is located in the human chromosome at 8p23.1. Cathepsin B is a lysosomal proteolytic enzyme of cysteine protease family. It is present in all mammalian cells. It is essential for the intracellular protein turnover.

Anwendung

Cathepsin B from human placenta has been used in the in vitro digestion of modified myelin oligodendrocyte glycoprotein (MOG). It has also been used in determining the effect of cysteine protease inhibitors.
Cathepsin B is a lysosomal cysteine proteinase which hydrolyzes proteins with a broad specificity for peptide bonds. Cathepsin B may be a useful tool in Alzheimer′s research, as it may have a role in the natural defense against the disease . Cathepsin B may be used to cleave procaspase 1 and procaspase 11, and to induce apoptosis in digitonin-permeabilized cells.

Biochem./physiol. Wirkung

Cathepsin B preferentially cleaves at the caboxyl side of Arg-Arg bonds in small molecule substrates. Cathepsin B has been shown to degrade β-amyloid precursor protein into harmless fragments . Cathepsin B inhibition has been shown to limit bone metastasis in breast cancer .
Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Einheitendefinition

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

Physikalische Form

Lyophilized powder containing phosphate buffer salts

Angaben zur Herstellung

Dissolves in water at 1 mg/mL concentration, yieliding a clear solution.

Inhibitor

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Beschreibung
Preisangaben

Substrat

Produkt-Nr.
Beschreibung
Preisangaben

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Cathepsins B, H, L and cysteine protease inhibitors in malignant prostate cell lines, primary cultured prostatic cells and prostatic tissue
Friedrich B, et al.
European Journal of Cancer, 35(1), 138-144 (1999)
Viktorija Herceg et al.
Molecules (Basel, Switzerland), 25(18) (2020-09-24)
Cyclopeptidic chemotherapeutic prodrugs (cPCPs) are macromolecular protease-sensitive doxorubicin (DOX) prodrugs synthesized from a cyclodecapeptidic scaffold, termed Regioselectively Addressable Functionalized Template (RAFT). In order to increase the chemotherapeutic potential of DOX and limit its toxicity, we used a Cathepsin B (Cat
Mixed gastric carcinomas show similar chromosomal aberrations in both their diffuse and glandular components.
Carvalho B, et al.
Analytical Cellular Pathology, 28(5-6), 283-294 (2006)
Scavenger Receptor A Mediates the Clearance and Immunological Screening of MDA-Modified Antigen by M2-Type Macrophages
Warnecke A, et al.
Neuromolecular Medicine, 19(4), 463-479 (2017)
Sarah Mueller-Steiner et al.
Neuron, 51(6), 703-714 (2006-09-20)
Alzheimer's disease (AD) may result from the accumulation of amyloid-beta (Abeta) peptides in the brain. The cysteine protease cathepsin B (CatB) is associated with amyloid plaques in AD brains and has been suspected to increase Abeta production. Here, we demonstrate

Artikel

This procedure applies to all products that have a specification for Cathepsin B activity determined by the liberation of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin.

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