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P5459

Sigma-Aldrich

Protease aus Bacillus licheniformis

glycerol solution (50%)

Synonym(e):

Alkalase, Proteinase aus Bacillus licheniformis, Subtilisin A, Subtilisin Carslberg

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About This Item

CAS-Nummer:
9014-01-1
EC-Nummer:
3.4.21.62 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00132092
UNSPSC-Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

Biologische Quelle

Bacillus licheniformis

Qualitätsniveau

200

Form

glycerol solution (50%)

Spezifische Aktivität

≥7 units/mg protein (biuret)

Konzentration

≥50 mg/mL

Löslichkeit

0.85% sodium chloride: soluble, clear (Diluted)
H2O: miscible

Lagertemp.

2-8°C

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Allgemeine Beschreibung

The enzyme hydrolyzes gelatin optimally at 50 °C over the range of pH 8-10. It is stable between pH 6-10 at 50 °C. Commercially, it is used in laundry detergent formulations and also for the recovery of silver from photographic emulsions.

Anwendung

Protease from Bacillus licheniformis has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens.
Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Protease from Bacillus licheniformis, product P5459, has been used to hydrolyze muscle during the isolation of mitochondria from skeletal muscle.

Biochem./physiol. Wirkung

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.

Verpackung

Package size based on protein content.

Einheitendefinition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Piktogramme

Health hazardCorrosion
GHS08,GHS05

Signalwort

Danger

Gefahreneinstufungen

Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Analysenzertifikate (COA)

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Rachel A Schlaak et al.
Frontiers in cardiovascular medicine, 7, 23-23 (2020-03-21)
Radiation therapy is received by over half of all cancer patients. However, radiation doses may be constricted due to normal tissue side effects. In thoracic cancers, including breast and lung cancers, cardiac radiation is a major concern in treatment planning.
H T Skiri et al.
Chemical senses, 29(3), 253-267 (2004-03-30)
The primary olfactory centre, the antennal lobe of Heliothis virescens moths, contains 62 glomeruli which process plant odour information and four male-specific glomeruli which form the macroglomerular complex, involved in processing information about pheromone and interspecific signals. Using calcium imaging
Karima Ait-Aissa et al.
Scientific reports, 9(1), 7623-7623 (2019-05-22)
Coronary artery disease (CAD) is a leading cause of death worldwide and frequently associated with mitochondrial dysfunction. Detailed understanding of abnormalities in mitochondrial function that occur in patients with CAD is lacking. We evaluated mitochondrial damage, energy production, and mitochondrial
Erin L Seifert et al.
The Journal of biological chemistry, 283(37), 25124-25131 (2008-07-17)
Uncoupling protein-3 (UCP3) is a mitochondrial inner membrane protein expressed most abundantly in skeletal muscle and to a lesser extent in heart and brown adipose tissue. Evidence supports a role for UCP3 in fatty acid oxidation (FAO); however, the underlying
H Ikemura et al.
The Journal of biological chemistry, 262(16), 7859-7864 (1987-06-05)
Subtilisin E, an alkaline serine protease of Bacillus subtilis 168, is first produced as a precursor, pre-pro-subtilisin, which consists of a signal peptide for protein secretion (pre-sequence) and a peptide extension of 77 amino acid residues (pro-sequence) between the signal
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Protokolle

Enzymatic Assay of Protease Using Casein As a Substrate

To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.

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