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P4630

Sigma-Aldrich

Protease aus Rinderpankreas

Type I, ≥5 units/mg solid

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About This Item

CAS-Nummer:
9001-92-7
EG-Nummer:
232-642-4
MDL-Nummer:
MFCD00132092
UNSPSC-Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

Biologische Quelle

bovine pancreas

Qualitätsniveau

200

Typ

Type I

Form

powder

Spezifische Aktivität

≥5 units/mg solid

Lagertemp.

−20°C

Anwendung

Non-specific Protease Activity Assay Video
Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Product P4630 is from bovine pancreas. Protease from bovine pancrease (type I) has been used for the extraction of hemicellulose.

Biochem./physiol. Wirkung

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.

Qualität

Crude

Einheitendefinition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

Piktogramme

Health hazardExclamation mark
GHS08,GHS07

Signalwort

Danger

Gefahreneinstufungen

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
SLCL2570
SLBW6516
SLBW7853
SLBT6188
056K7690

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Protokolle

Universal Protease Activity Assay: Casein as a Substrate

Proteases break peptide bonds. In the lab, it is often necessary to measure and/or compare the activity of proteases. Sigma's non-specific protease activity assay may be used as a standardized procedure to determine the activity of proteases, which is what we do during our quality control procedures.

Enzymatic Assay of Protease Using Casein As a Substrate

To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.

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