324708

Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi

• Synonym(e): Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi
• CAS-Nummer: 9001-92-7
• EC-Nummer: 3.4.24.33 (BRENDA, IUBMB)
• UNSPSC-Code: 12352202
• NACRES: NA.54

Eigenschaften

• Qualität: Proteomics Grade
• Qualitätsniveau: 100
• Assay: ≥90% (SDS-PAGE)
• Form: lyophilized solid
• Spezifische Aktivität: ≥20,000 units/mg protein
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Farbe: white
• Löslichkeit: water: soluble
• Versandbedingung: ambient
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins and the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK.

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Warnhinweis

Toxicity: Harmful (C)

Einheitendefinition

One unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.

Physikalische Form

Lyophilized from 10 mM Tris-HCl, pH 7.5.

Rekonstituierung

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Sonstige Hinweise

Drapeau, G.R. 1980. J. Biol. Chem.255, 839.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Piktogramme: GHS08,GHS07
• Signalwort: Danger
• H-Sätze: H315,H319,H334,H335
• P-Sätze: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Gefahreneinstufungen: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1