76546

Lipase, immobilisiert auf Immobead 150 aus Thermomyces lanuginosus

≥3000 U/g

• Synonym(e): Immobilized lipase, Lipase enzyme
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: fungus (Thermomyces lanuginosus)
• Form: powder (or beads)
• Spezifische Aktivität: ≥3000 U/g
• Methode(n): analytical sample preparation: suitable
• Farbe: white to off-white
• Anwendung(en): life science and biopharma
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Research area: Cell Signaling

Lipase from Thermomyces lanuginosus (TLL) is a basophilic, single-chain protein and thermostable enzyme. It belongs to the alpha/beta-hydrolase fold family. TLL is roughly spherical and is made up of a central seven-stranded β-parallel sheet with five interconnecting α-helices.

Anwendung

Lipase, immobilized on Immobead 150 from Thermomyces lanuginosus has been used:

• as a biocatalyst to study ultrasound-assisted transesterification of a blend of non-edible oils with methanol.
• as a catalyst in the synthesis of medium chain fatty acids (MCFA) and polyunsaturated fatty acids (PUFA) rich oils by enzymatic structuring of flax seed oil.
• for the resolution of chiral compounds and the transesterification production of biodiesel.
• to enhance the efficiency and selectivity of the enzymatic synthesis of ethyl-(R)-2-hydroxy-4-phenylbutyrate, (R)-HPB ester.
• to facilitate a chemoenzymatic process for the continuous synthesis of (S)-7-((tert-Butyldiphenylsilyl)oxy)hept-1-yn-4-ol, a crucial intermediate for Eribulin synthesis, within a packed-bed reactor setup.

Biochem./physiol. Wirkung

It participates in catalyzing the hydrolysis of triglycerides at the water-lipid interface and produces mono-, di-glycerides, and free fatty acids. Due to its versatility, this enzyme is applicable in various industries including, detergents, paper, leather, baking, fine chemicals, fuel, and biocatalysts.

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Einheitendefinition

1 U entspricht der Enzymmenge, die 1 μmol Buttersäure pro Minute bei pH 10.0 und 40°C freisetzt (Tributyrin, Fluka^®-Nr. 91010, als Substrat)

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)