62288

Lipase B Candida antarctica, rekombinant aus Aspergillus oryzae

powder, beige, ~9 U/mg

• CAS-Nummer: 9001-62-1
• EC-Nummer: 3.1.1.3 (BRENDA, IUBMB)
• EG-Nummer: 232-619-9
• MDL-Nummer: MFCD00131509
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Rekombinant: expressed in Aspergillus oryzae
• Qualitätsniveau: 100
• Form: powder
• Spezifische Aktivität: ~9 U/mg
• Mol-Gew.: 33 kDa
• Farbe: beige
• Lagertemp.: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChIKey: QWZUIMCIEOCSJF-CHHCPSLASA-N

Beschreibung

Allgemeine Beschreibung

Candida antarctica lipase B (CalB) is structurally similar to several other lipases and has a flexible lid. It is made up of 317 amino acids and has a molecular weight of 33 kDa. Lipase B is a member of the alpha/beta hydrolase-fold family.

Anwendung

Lipase B Candida antarctica, recombinant from Aspergillus oryzae has been used:

• as a standard to characterize the enzymatic properties of D5-CalB
• as an efficient biocatalyst to start the reaction to obtain (R)-ester via esterification of racemic secondary alcohol
• to investigate a “green” recycling route for polybutylene succinate (PBS) based on reactive extrusion
• to compare the esterification yield with adsorbed CaLB (aCaLB) and covalently immobilized CaLB (cCaLB)

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem./physiol. Wirkung

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.
Candida antarctica lipase B (CALB) possesses wide substrate specificity, high activity and high enantioselectivity, hence it is considered as a major enzyme in biotechnology. It also has the capability to perform in aqueous and non-aqueous reaction environments. CALB is used in transesterification, kinetic resolution and polymerization reactions.

Lipase B from Candida antarctica has been shown to be an effective catalyst for the synthesis of esters of ethyl D-glucopyranoside from fatty acids larger than octanoic acid. It has also been found to catalyze a wide variety of organic reactions including many different regio- and enantio-selective syntheses.

Einheitendefinition

1 U entspricht der Enzymmenge, die 1 μmol Buttersäure pro Minute bei pH 8.0 und 40°C freisetzt (Tributyrin, Fluka^®-Nr. 91010, als Substrat)

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)