MAB1683
Anti-Ankyrin Antibody, clone Ank016
clone Ank016, Chemicon®, from mouse
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About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified immunoglobulin
Antikörper-Produkttyp
primary antibodies
Klon
Ank016, monoclonal
Speziesreaktivität
rat, human, rabbit, pig, mouse, bovine, canine
Hersteller/Markenname
Chemicon®
Methode(n)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotyp
IgG2a
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... ANK1(286)
Verwandte Kategorien
Spezifität
Reacts with the intracellular ankyrin (216 kDa). Ankyrin consists of a single peptide of M.W. 215 kDa known to be involved in the attachment of spectrin containing membrane skeleton to the plasma membranes. Ankyrin belongs to a family of closely related polypeptides associated with the plasma membrane of cells in a variety of cell types (e.g., lymphocytes, platelets, fibroblasts and endothelial cells) and various tissues (e.g., brain, kidney, muscle and many epithelial tissues).
Immunogen
Purified human erythroid cells ankyrin
Anwendung
Research Category
Zellstruktur
Zellstruktur
Research Sub Category
Cytoskelett
Cytoskelett
Anti-Ankyrin Antibody, clone Ank016 is an antibody against Ankyrin for use in ELISA, FC, IP, WB & IC.
Western blotting at 1:200; low percentage PAGE gels a must; protein is quite large; proteinase inhibitors recommended or lower level fragments likely; membrane preparations and enhance signals.
ELISA: 1:1000
Immunoprecipitation: 1:100
Immunocytochemistry, using 2% paraformaldehyde fixation; higher fixation (i.e. 4% PFA can be problematic) 1:100; light fixation 5-10′ RT usually; permeabilize lightly with 0.1% triton X-100 as protein is intracellular.
Flow cytometry: 1:500
Immunohistochemistry: 1:100; light fixations suggested; PLP or mild zinc formalin recommended.
RIA: 1:5000
Optimal working dilutions must be determined by end user.
ELISA: 1:1000
Immunoprecipitation: 1:100
Immunocytochemistry, using 2% paraformaldehyde fixation; higher fixation (i.e. 4% PFA can be problematic) 1:100; light fixation 5-10′ RT usually; permeabilize lightly with 0.1% triton X-100 as protein is intracellular.
Flow cytometry: 1:500
Immunohistochemistry: 1:100; light fixations suggested; PLP or mild zinc formalin recommended.
RIA: 1:5000
Optimal working dilutions must be determined by end user.
Physikalische Form
Format: Purified
Purified immunoglobulin from culture supernatant. Liquid in 10 mM sodium phosphate, pH 7.5 and 150mM NaCl with 0.01% NaN3.
Lagerung und Haltbarkeit
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Sonstige Hinweise
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
P A Lang et al.
Cell death and differentiation, 12(5), 415-428 (2005-03-05)
Hyperosmotic shock, energy depletion, or removal of extracellular Cl(-) activates Ca(2+)-permeable cation channels in erythrocyte membranes. Subsequent Ca(2+) entry induces erythrocyte shrinkage and exposure of phosphatidylserine (PS) at the erythrocyte surface. PS-exposing cells are engulfed by macrophages. The present study
Spectrin-based membrane skeleton: a multipotential adaptor between plasma membrane and cytoplasm.
Bennett, V
Physiological Reviews, 70, 1029-1065 (1990)
The lymphoma transmembrane glycoprotein GP85 (CD44) is a novel guanine nucleotide-binding protein which regulates GP85 (CD44)-ankyrin interaction.
Lokeshwar, V B and Bourguignon, L Y
The Journal of Biological Chemistry, 267, 22073-22078 (1992)
Capping and the cytoskeleton.
Bourguignon, L Y and Bourguignon, G J
International Review of Cytology, 87, 195-224 (1984)
L Y Bourguignon et al.
Journal of immunology (Baltimore, Md. : 1950), 151(12), 6634-6644 (1993-12-15)
The purposes of this study are to characterize the binding of hyaluronic acid (HA) to mouse T lymphoma cells, to measure changes in intracellular Ca2+ after HA binding, to elucidate the interaction between the HA receptor, GP85(CD44), and ankyrin in
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