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HPLC Analysis of Paclitaxel and Related Compounds on Ascentis® Express F5

HPLC Analysis of Paclitaxel and Related Compounds on Ascentis® Express F5 application for HPLC

Materiali

Colonna analitica

N° Catalogo
Descrizione
Determinazione del prezzo

50631-U

Ascentis® Express F5, 5 μm HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

Standard

N° Catalogo
Descrizione
Determinazione del prezzo

B8154

Baccatin III

≥95% (HPLC)

T1912

Paclitaxel

from Taxus yannanensis, powder

C4991

Cephalomannine

≥97% (HPLC), solid

CONDITIONS

column

Ascentis Express F5, 15 cm x 4.6 mm I.D., 5 μm particles (50631-U)

mobile phase

[A] water; [B] acetonitrile; (60:40, A:B)

flow rate

1.5 mL/min

pressure

2727 psi (188 bar)

column temp.

30 °C

detector

UV, 227 nm

injection

5 μL

sample

taxols 25 μg/mL in 70:15:15, water:methanol:acetonitrile

Descrizione

Risultati analitici

An efficient reverse phase HPLC method for the HPLC analysis of of Paclitaxel (Taxol) and related compounds is developed using the Ascentis® Express F5 5μm Fused Core HPLC column. Paclitaxel is a natural product with antitumor activity. TAXOL (paclitaxel) is obtained via a semi-synthetic process from Taxus baccata.
The developed method can be easily used for the routine quality control of bulk and Parenterals dosage form of Paclitaxel within a short analysis time. This method offers the advantages of higher efficies, reduced backpressure and shorter run times over a traditionally run methods over 5 μm totally porous particle columns.

Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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