HPLC Analysis of Asparagine Enantiomers on Astec® CHIROBIOTIC® T (25 °C)
Materiali
Colonna analitica
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Precolonna
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Astec® Chirobiotic® T Chiral HPLC Guard Column
Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mmStandard
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L-Asparagine
certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, SwitzerlandCONDITIONS
column
CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase
[A] water); [B] methanol; [C] formic acid; (30:70:0.02, A:B:C)
flow rate
1 mL/min
detector
UV, 205 nm
column temp.
25 °C
injection
10 μL
sample
300 μg /mL in 30:70 water:methanol
Descrizione
Note legali
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC