Passa al contenuto

Quantification of 3-MCPD and its mercapturic metabolite in human urine: validation of an LC-MS-MS method and its application in the general population.

Quantification of 3-MCPD and its mercapturic metabolite in human urine: validation of an LC-MS-MS method and its application in the general population.

Analytical and bioanalytical chemistry (2015-04-12)

Roberta Andreoli, Martina Cirlini, Antonio Mutti

ABSTRACT

A new method for the simultaneous quantitative determination in human urine of 3-monochloropropane-1,2-diol (3-MCPD), a toxic food contaminant, and its metabolite, 2,3-dihydroxypropyl mercapturic acid (DHPMA), was developed and validated. After urine dilution, the analytes were separated on an Atlantis®dC18 column and quantified by liquid chromatography-tandem mass spectrometry using isotopically labelled internal standards. The limits of quantification (S/N = 10) were 1.90 and 2.21 μg/L for 3-MCPD and DHPMA, respectively. Intra- and inter-day precision were lower than 6 % for each compound. Matrix effects were evaluated. Due to the high sensitivity and good accuracy of the method, 3-MCPD and DHPMA were found in 67 and 100 % of urine samples of healthy subjects, respectively.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

Sigma-Aldrich

Acido solforico, 99.999%

Sigma-Aldrich

Trietilammina, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

Acetonitrile, anhydrous, 99.8%

Sigma-Aldrich

L-Cisteina, anhydrous, ≥98% (TLC)

Sigma-Aldrich

Acido acetico, for luminescence, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

Trietilammina, ≥99.5%

Sigma-Aldrich

Metanolo, anhydrous, 99.8%

Sigma-Aldrich

1-butanolo, for molecular biology, ≥99%

Sigma-Aldrich

L-Cisteina, anhydrous, ≥99.0% (RT)

Sigma-Aldrich

Acido acetico, ≥99.5%, FCC, FG

Sigma-Aldrich

Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%

Sigma-Aldrich

Acido acetico, natural, ≥99.5%, FG

Sigma-Aldrich

(±)-3-Chloro-1,2-propanediol, 98%

Sigma-Aldrich

L-Cisteina, anhydrous, from non-animal source, BioReagent, suitable for cell culture, ≥98.0%

Sigma-Aldrich

Trietilammina, ≥99%

Sigma-Aldrich

Acetic anhydride, 99.5%

Sigma-Aldrich

Acetic anhydride, ReagentPlus^®, ≥99%

Sigma-Aldrich

Acetone, natural, ≥97%

Sigma-Aldrich

1-butanolo, natural, ≥99.5%, FCC, FG

Sigma-Aldrich

Acetone, ≥99%, meets FCC analytical specifications

Sigma-Aldrich

Acetonitrile, electronic grade, 99.999% trace metals basis

Sigma-Aldrich

Trietilammina, ≥99.5%

Sigma-Aldrich

1-butanolo, anhydrous, 99.8%

Sigma-Aldrich

Trietilammina, for protein sequence analysis, ampule, ≥99.5% (GC)

Sigma-Aldrich

5α-Androstan-17β-ol-3-one, purum, ≥99.0% (TLC)

Sigma-Aldrich

Acetic anhydride, ACS reagent, ≥98.0%

Sigma-Aldrich

Trietilammina, for amino acid analysis, ≥99.5% (GC)

Sigma-Aldrich

Trietilammina, puriss. p.a., ≥99.5% (GC)

Sigma-Aldrich

Metanolo, NMR reference standard

Sigma-Aldrich

Acetic anhydride, Arxada quality, ≥99.5% (GC)