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WGA1

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GenomePlex® Whole Genome Amplification (WGA) Kit

Kit for whole genome amplification from a variety of DNA sources including FFPE tissue

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About This Item

Codice UNSPSC:
41121800
NACRES:
NA.55

Livello qualitativo

200

tecniche

whole genome amplification: suitable

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Descrizione generale

GenomePlex® Whole Genome Amplification WGA kit is suitable for use with genomic DNA, whole blood, buffy coats, buccal swabs, and cultured cells. Amplification requires only a small amount of starting material (1 μL blood, single cheek swab, or 10 ng of gDNA), which after PCR produces a yield of 5 - 10 μg per reaction. The amplified DNA can be purified and stored or used for downstream analyses including ABI′s TaqMan® assays, microsatellite analysis, or sequencing. GenomePlex gives a complete representation of the entire genome with no detectable allele bias.

WGA Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.

GenomePlex method allows the researcher to generate a representative, ~500-fold amplification of genomic DNA. The amplification yield is dependent on the purity and amount of starting material. This kit contains all the required reagents to perform library preparation and fragmentation.

Applicazioni

GenomePlex® Whole Genome Amplification (WGA) Kit has been used to amplify input and chromatin immunoprecipitation (ChIP) DNA. It has also been used in the fragmentation of bacterial artificial chromosome (BAC) clones from bacterial cultures for the preparation of repeat free (RF) probes.

Caratteristiche e vantaggi

  • Higher yield from minimal template: Amplification of nanogram amounts (10ng) of genomic DNA to microgram yields (5-10 μg) in less than about three hours
  • Nanograms of samples can be preserved at –20 °C for future use
  • Choose from a variety of DNA sources: whole blood, buccal swab, blood card, plant, soil, and formalin-fixed paraffin-embedded tissue (FFPE)
  • Compatible with a wide variety of downstream applications such as TaqMan® and BeadArray assays
  • Universal primers
  • Whole-genome representation with no detectable bias
  • Increased accuracy in amplification produces no amplicon in the negative control reactions

Altre note

The sequences of the universal primers provided in this kit are considered proprietary.

Note legali

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • W4502Water, Nuclease-Free Water, for Molecular BiologySDS

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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Adam Hittelman et al.
Diagnostic molecular pathology : the American journal of surgical pathology, part B, 16(4), 198-206 (2007-11-29)
Genome-based technologies such as genomic arrays and next generation sequencing are poised to make significant contributions to clinical oncology. However, translation of these technologies to the clinic will require that they produce high-quality reproducible data from small archived tumor specimens
Amy Breman et al.
Prenatal diagnosis, 32(4), 351-361 (2012-04-03)
To evaluate the results of prenatal chromosomal microarray analysis (CMA) on >1000 fetal samples referred for testing at our institution and to compare these data to published reports. High resolution CMA was offered to women undergoing amniocentesis or chorionic villus
Kaye N Ballantyne et al.
Forensic science international, 166(1), 35-41 (2006-05-12)
The analysis of LCN or highly degraded DNA samples presents a challenge for forensic science. Improving the quantity and/or quality of samples would greatly increase the profiling success rate from LCN and degraded samples. Whole genome amplification (WGA) is one
Nicola A Johnson et al.
Laboratory investigation; a journal of technical methods and pathology, 86(9), 968-978 (2006-06-06)
Array-based comparative genomic hybridisation (aCGH) has diverse applications in cancer gene discovery and translational research. Currently, aCGH is performed primarily using high molecular weight DNA samples and its application to formalin-fixed and paraffin-embedded (FFPE) tissues remains to be established. To
Binding patterns of BCL11A in the globin and GATA1 loci and characterization of the BCL11A fetal hemoglobin locus
Jawaid K, et al.
Blood Cells, Molecules and Diseases, 45(2), 140-146 (2010)
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Protocolli

Whole Gene Amplification Formalinfixed Paraffin embedded Tissues

Archived formalin-fixed and paraffin-embedded (FFPE) tissue collections represent invaluable resources for studying pathogenesis of cancer and a variety of other diseases.

Protocol for DNA extraction & WGA Amplification from Soil Samples

Genomic DNA from soil samples can be easily damaged by nucleases and contaminating debris resulting in low DNA yields. As a result, the researcher’s ability to perform downstream analysis may be compromised. After isolating DNA from the soil sample, the GenomePlex® Whole Genome Amplification Protocol is followed

GenomePlex Whole Genome Amplification Kit Protocol (WGA1)

GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA

Saliva DNA Extraction & WGA Amplification Protocol

This protocol provides a simple and convenient method to isolate, amplify and purify genomic DNA from saliva

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Contenuto correlato

GenomePlex Whole Genome Amplification Kit Protocol (WGA1)

GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA

Animal Tissue DNA-Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.

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