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W0876

Sigma-Aldrich

White′s Basal Salt Mixture

powder, suitable for plant cell culture

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.72

Stato

powder

Livello qualitativo

tecniche

cell culture | plant: suitable

applicazioni

agriculture

Temperatura di conservazione

2-8°C

Descrizione generale

White′s Basal Salt Mixture is widely used in plant tissue culture.

Applicazioni

White′s Basal Salt Mixture has been used:
  • as a medium for the precultured spores of Prothalli of Adiantum capillus for the generation of protonemata
  • to promote germination and cotton cotyledon unfolding
  • as a solidified medium component for culturing A. capillus and as a component of selection medium for gametophyte selection after particle bombardment

Formula variabile

With the macro- and micronutrients as described by White (1968).

Media Formulation

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Aquatic Chronic 2 - Eye Dam. 1 - Ox. Sol. 3 - STOT RE 2 Inhalation

Organi bersaglio

Brain

Codice della classe di stoccaggio

5.1B - Oxidizing hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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K Rajasekaran
Methods in molecular biology (Clifton, N.J.), 1902, 35-45 (2018-12-14)
Biolistic transformation of cotton (Gossypium hirsutum L.) meristems, isolated from mature seed, is detailed in this report. A commercially available, helium-driven biolistic device (Bio-Rad PDS1000/He ) was used to bombard gold particles coated with a marker gene (uidA or "GUS")
S J Sata et al.
Methods in cell science : an official journal of the Society for In Vitro Biology, 22(4), 299-304 (2001-09-11)
An efficient novel method of direct somatic embryogenesis from basal tissue of garlic clove was developed. The influence of plant growth regulators, basal medium and explant type on somatic embryo induction was examined. The best plant growth regulator combination was
W Bressan et al.
Canadian journal of microbiology, 46(8), 741-743 (2000-08-15)
Responses of somatic embryos of sweet potato (Ipomoea batata (L.) Poir., cv. White Star) at different developmental stages to in vitro inoculation with Glomus etunicatum (Becker and Gerdemann) (isolate INVAM FL329) were evaluated. Somatic embryos were grown in glass tubes
S Chattopadhyay et al.
Applied microbiology and biotechnology, 60(5), 541-546 (2003-01-22)
The effect of major medium ingredients (sugar, nitrogen source and phosphate) in Podophyllum hexandrum suspension cultures was investigated in order to increase the production of podophyllotoxin, the raw material in the synthesis of anticancer drugs. Amongst B5, Eriksson, MS, Nitsch
D Parfitt et al.
Journal of experimental botany, 55(406), 2169-2177 (2004-09-14)
If, following an inductive treatment of 2 d of continuous darkness, shoot apices of Pharbitis nil are cultured 1 d later on White's medium supplemented with 2% sucrose, they cannot form carpels, but they can if they are cultured on

Articoli

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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