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Key Documents

V2258

Sigma-Aldrich

Monoclonal Anti-Vimentin antibody produced in mouse

clone LN-6, ascites fluid

Sinonimo/i:

Anti-F5H288

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

ascites fluid

Tipo di anticorpo

primary antibodies

Clone

LN-6, monoclonal

contiene

15 mM sodium azide

Reattività contro le specie

pig, mouse, sheep, human, bovine, rabbit, feline, rat

tecniche

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue sections
immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable using cultured cells
western blot: suitable

Isotipo

IgM

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... VIM(7431)
mouse ... Vim(22352)
rat ... Vim(81818)

Descrizione generale

Vimentin belongs to the class of type III intermediate filament protein family. The gene is located on human chromosome 10p13. It is a cytoskeletal protein. Vimentin is mainly expressed in normal mesenchymal cells.
Vimentin is a 58 kD class III intermediate filament present mainly in mesenchymal cells. It regulates cell shape, motility and endurance of mechanical stress in mesenchymal cells. Monoclonal anti-vimentin antibody can be used for the localization of vimentin by various immunochemical assays like immunoblotting, immunohistochemistry and immunoprecipitation. Frozen B5 fixed and formalin fixed, paraffin embedded tissue sections can be stained using anti-vimentin antibody. Mouse monoclonal anti-vimentin antibody reacts specifically with an epitope of the cytoskeletal protein vimentin (58kD). The product shows cross reactivity with vimentin from bovine, human, pig, sheep, cat, rat, mouse and rabbit.

Specificità

Recognizes an epitope of the cytoskeleton protein vimentin that is not detected in human lymphoid cells or derived malignancies. It is therefore useful in differentiating sarcomas from lymphomas.

Immunogeno

human thymic nuclear extract

Applicazioni

Monoclonal Anti-Vimentin antibody can be used in immunocytochemistry and immunohistochemical staining. It can also be used in immunofluorescence staining (diluted 1: 500) .
Monoclonal Anti-Vimentin antibody produced in mouse has been used in western blotting and immunolabeling.

Azioni biochim/fisiol

Vimentin plays an important role in modulating cell growth. It plays a pivotal role in epithelial-mesenchymal transition. Vimentin acts as a potential target for anti-metastasis therapy for nasopharyngeal carcinoma (NPC). It regulates cellular architecture and tissue integrity. It is also involved in tumorigenesis and the metastatic spread of cancer. Vimentin provides stress resistance. It might be involved in neuritogenesis.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Raccomandato

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Margit A Huber et al.
The Journal of clinical investigation, 114(4), 569-581 (2004-08-18)
The transcription factor NF-kappaB is activated in a range of human cancers and is thought to promote tumorigenesis, mainly due to its ability to protect transformed cells from apoptosis. To investigate the role of NF-kappaB in epithelial plasticity and metastasis
Fractional CO2 laser: a novel therapeutic device upon photobiomodulation of tissue remodeling and cytokine pathway of tissue repair
Prignano F, et al.
Dermatologic Therapy, 22, S8-S15 (2009)
Alternatively spliced lysyl oxidase-like 4 isoforms have a pro-metastatic role in cancer
Sebban S, et al.
Clinical & Experimental Metastasis, 30(1), 103-117 (2013)
M J Gibson et al.
Endocrinology, 141(9), 3065-3071 (2000-08-31)
Axons of GnRH neurons terminate at the median eminence in the medial basal hypothalamus (MBH) of the brain early in development. Similarly, GnRH neurons in grafts of preoptic area (POA) tissue within the third ventricle of hypogonadal mice preferentially innervate
Vimentin is a crucial target for anti-metastasis therapy of nasopharyngeal carcinoma
Wang W, et al.
Molecular and Cellular Biochemistry, 438(1-2), 47-57 (2018)

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