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Key Documents

T9822

Sigma-Aldrich

Anti-Tubulin, Polyglutamylated antibody, Mouse monoclonal

clone B3, purified from hybridoma cell culture

Sinonimo/i:

Anti-K-ALPHA-1

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

B3, monoclonal

Forma fisica

buffered aqueous solution

PM

antigen ~50 kDa

Reattività contro le specie

mouse, sea urchin (Lytechinus pictus and three others.), bovine, monkey, rabbit, human, rat, Oxyrrhis marina (dinoflagellate), chicken

tecniche

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using cytosolic fraction of rat brain extract

Isotipo

IgM

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

Descrizione generale

Tubulin is a 100kD protein that is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-tubulin, polyglutamylated antibody can be used as primary antibody in colocalization experiments. This antibody can also be used for studies involving the inhibition of flagellar motility in permeabilized sperm models. Mouse anti-tubulin, polyglutamylated antibody reacts specifically with the epitope present in the C-terminal region of α and β tubulins (glutamylated motif at amino acids 445-457 of α-tubulin).
Tubulin is a cellular colchicine-binding protein.

Immunogeno

purified sea urchin (Lytechinus pictus) sperm axonemal proteins.

Applicazioni

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Tubulin, Polyglutamylated antibody produced in mouse has been used in:
  • immunoprecipitation
  • immunohistochemistry
  • immunofluorescence

Monoclonal anti-tubulin, polyglutamylated antibody can be used in immunoblotting, immunocytochemistry and ELISA. It can also be used as primary antibodies (diluted 1:1000) in western blotting.

Azioni biochim/fisiol

Microtubule plays an important role in DNA segregation.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Parathyroid hormone-related peptide represses chondrocyte hypertrophy through a protein phosphatase 2A/histone deacetylase 4/MEF2 pathway
Kozhemyakina E, et al.
Molecular and Cellular Biology, 29(21), 5751-5762 (2009)
A Mutation in CCDC50, a Gene Encoding an Effector of Epidermal Growth Factor-Mediated Cell Signaling, Causes Progressive Hearing Loss
Modamio-HoybjorS, et al.
American Journal of Human Genetics, 80(6), 1076-1089 (2007)
Mitotic spindle assembly and chromosome segregation: refocusing on microtubule dynamics
Kline-Smith SL and Walczak CE
Molecular Cell, 15(3), 317-327 (2004)
SDCCAG8 interacts with RAB effector proteins RABEP2 and ERC1 and is required for hedgehog signaling
Airik R, et al.
PLoS ONE, 11(5), e0156081-e0156081 (2016)
Insight into tubulin regulation from a complex with colchicine and a stathmin-like domain
Ravelli RBG, et al.
Nature, 428(6979), 198-198 (2004)

Articoli

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.