T9784

Tricina

BioXtra, pH 4.0-6.0 (1 M in H₂O), ≥99% (titration)

• Sinonimo/i: N-[tris(idrossimetil)metil]glicina
• Formula condensata: (HOCH₂)₃CNHCH₂CO₂H
• Numero CAS: 5704-04-1
• Peso molecolare: 179.17
• Beilstein: 1937804
• Numero CE: 227-193-6
• Numero MDL: MFCD00004277
• Codice UNSPSC: 12161700
• ID PubChem: 24900603
• NACRES: NA.25

Proprietà

• Nome Commerciale: BioXtra
• Livello qualitativo: 400
• Saggio: ≥99% (titration)
• Forma fisica: powder
• Impurezze: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter
• Residuo alla calcinazione: ≤0.1%
• pH: 4.0-6.0 (1 M in H₂O)
• Intervallo di pH utile: 7.4-8.8
• pKa (25 °C): 8.1
• Solubilità: H₂O: 1 M, clear, colorless
• Anioni in tracce: chloride (Cl^-): ≤0.1%; sulfate (SO₄^2-): ≤0.05%
• Cationi in tracce: Al: ≤0.0005%; Ca: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Na: ≤0.005%; Pb: ≤0.001%; Zn: ≤0.0005%
• Assorbimento: ≤0.05 at 280 in H₂O at 1 M; ≤0.1 at 260 in H₂O at 1 M
• applicazioni: diagnostic assay manufacturing
• Stringa SMILE: OCC(CO)(CO)NCC(O)=O
• InChI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12); SEQKRHFRPICQDD-UHFFFAOYSA-N

Descrizione

Descrizione generale

The name Tricine is derived from "tris" and "glycine". Tricine and other good buffers serve as efficient scavengers of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is widely used as an electrophoresis buffer and for the resuspension of cell pellets. Due to its lower negative charge compared to glycine, it migrates faster. It has a high ionic strength which leads to more ion movement and less protein movement. This aids the separation of low molecular weight proteins in lower percent acrylamide gels. Tricine is also used as a trailing ion that permits the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems.

Applicazioni

Tricine has been used:

• in a polymerase chain reaction (PCR) buffer used in the single-cell collection
• as a component of lactotroph feeding medium for culturing lactotrophs
• to prepare luciferase assay reagent for luciferase activity assay in firefly Photonis pyralis and as a component in Triton/glycylglycine lysis buffer

Componente del tampone per la separazione dei peptidi a basso peso molecolare.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 13 - Non Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)