T8694

TrkB/Fc Chimera human

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• Numero MDL: MFCD03458837
• Codice UNSPSC: 51111800
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Livello qualitativo: 200
• Ricombinante: expressed in NSO cells
• Saggio: >90% (SDS-PAGE)
• Stato: lyophilized powder
• Potenza: 0.1-0.4 μg/mL ED₅₀
• PM: 120-130 kDa by SDS-PAGE (reducing); calculated mol wt 71 kDa
• Confezionamento: pkg of 100 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• tecniche: western blot: suitable
• Impurezze: endotoxin, tested
• N° accesso UniProt: Q16620
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... NTRK2(4915)

Descrizione

Applicazioni

TrkB/Fc Chimera human can be used in synaptoneurosome preparation and western blot analysis. Intracerebroventricular administeration of TrkB/Fc chimera human blocks TrkB ligand signaling and was used to study the role of BDNF in ATN response augmentation.

Azioni biochim/fisiol

Binds specifically to mediate neurotrophins BDNF and NT-4/5.

Member of the Trk family tyrosine kinase receptors. Binds specifically to mediate neurotrophins BDNF and NT-4/5.

TrkB/Fc Chimera human consist of extracellular domain of human TrkB1 fused to histidine-tagged Fc region of human IgG1 by using a polypeptide linker. TrkB belongs to tyrosine kinase receptors family and facilitates the hippocampal development as well as regulating the synaptic plasticity that underlies long-term potentiation and learning. Brain derived neurotrophic factor (BDNF) and Neurotrophin 4 or 5 are preferred ligands for TrkB. Nevertheless it can also bind to NTF3/neurotrophin-3 that is less efficient in activating the receptor but regulates neuron survival through NTRK2.

Altre note

Extracellular domain of human TrkB fused to the C-terminal histidine-tagged Fc region of human IgG1.

Stato fisico

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline.

Ricostituzione

Reconstitute to 100 μg/mL in phosphate buffered saline.

Risultati analitici

Inhibits BDNF-induced proliferation of TrkB transfected cells, BaF-TrkB-BD.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable