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Key Documents

T4019

Sigma-Aldrich

Trypsin−Agarose

buffered aqueous suspension, from bovine pancreas trypsin

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About This Item

Numero MDL:
Codice UNSPSC:
12352204
eCl@ss:
42020142
NACRES:
NA.54

Origine biologica

bovine pancreas (trypsin)

Livello qualitativo

Forma fisica

buffered aqueous suspension

Concentrazione

≥15 units/mL (packed gel)

Grado di funzionalizzazione

≥15 units per mL (packed gel)

Matrice

beaded agarose

Temperatura di conservazione

2-8°C

Descrizione generale

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Applicazioni

An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.

Definizione di unità

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).

Stato fisico

Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative

Nota sulla preparazione

TPCK-treated

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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