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Documenti fondamentali

T1788

Sigma-Aldrich

Tyrode′s Solution, Acidic

liquid, sterile-filtered, suitable for mouse embryo cell culture

Sinonimo/i:

Cell culture solution

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.75

Sterilità

sterile-filtered

Livello qualitativo

Forma fisica

liquid

tecniche

cell culture | embryo: suitable

Impurezze

endotoxin, tested

pH

2.5±0.3

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Descrizione generale

Tyrode′s salt solution is used for diluting protein components of media of natural origin. The current role of a balanced salt solution in cell culture is multifaceted. It serves as an irrigating, transporting, and diluting agent while maintaining intra- and extracellular osmotic balance and provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It also provides the essential energy for cell metabolism and a buffering system to maintain the medium within the optimal pH range (7.2-7.6).

Applicazioni

Tyrode′s Solution, Acidic has been used for the removal of zona pellucida from oocytes/embryos.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Wenning Qin et al.
Genetics, 200(2), 423-430 (2015-03-31)
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system is an adaptive immune system in bacteria and archaea that has recently been exploited for genome engineering. Mutant mice can be generated in one step through direct delivery of
Zhao-Yang Xu et al.
Scientific reports, 5, 16978-16978 (2015-11-20)
Cep55 is a relatively novel member of the centrosomal protein family. Here, we show that Cep55 is expressed in mouse oocytes from the germinal vesicle (GV) to metaphase II (MII) stages. Immuostaining and confocal microscopy as well as time lapse
Sara Carvalhal et al.
Molecular biology of the cell, 28(19), 2470-2478 (2017-08-05)
Asymmetric cell divisions depend on the precise placement of the spindle apparatus. In mammalian oocytes, spindles assemble close to the cell's center, but chromosome segregation takes place at the cell periphery where half of the chromosomes are expelled into small
Liyan Sui et al.
Journal of cellular physiology, 235(10), 7030-7042 (2020-02-06)
Histone deacetylase 6 (HDAC6) participates in mouse oocyte maturation by deacetylating α-tubulin. However, how HDAC6 expression is regulated in oocytes remains unknown. In the present study, we discovered that mouse oocytes had a high level of HDAC6 expression and a
Jiangwei Lin et al.
Scientific reports, 6, 39457-39457 (2016-12-20)
Various types of stem cell lines have been derived from preimplantation or postimplantation mouse embryos: embryonic stem cell lines, epiblast stem cell lines, and trophoblast stem cell lines. It is not known if extraembryonic endoderm stem (XEN) cell lines can

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