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Key Documents

SRP6484

Sigma-Aldrich

Carbonic Anhydrase 2/CA2 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Sinonimo/i:

CA-II, CAII, Car2

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About This Item

Codice UNSPSC:
12352204
NACRES:
NA.32

Origine biologica

human

Ricombinante

expressed in HEK 293 cells

Tag

6-His tagged (C-terminus)

Saggio

≥95% (SDS-PAGE)

Forma fisica

lyophilized

PM

calculated mol wt 30 kDa
observed mol wt 30 kDa (DTT-reduced. Ser 2 is the predicted N-terminal .)

Confezionamento

pkg of 10 μg
pkg of 50 μg

Impurezze

<1 EU/μg endotoxin (LAL test)

N° accesso UniProt

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... CA2(760)

Descrizione generale

Carbonic Anhydrase 2 (CA2) is a zinc metalloenzyme and it helps in regulating acid-base balance in cells. It is a ubiquitous cytosolic protein. Carbonic anhydrase 2 also known as carbonate dehydratase II, carbonic anhydrase C, belongs to the class of a carbonic anhydrases. The associated gene is mapped to human chromosome 8q21.

Applicazioni

Carbonic Anhydrase 2/CA2 human has been used for CA2 ELISA (enzyme-linked immunosorbent assay).

Azioni biochim/fisiol

Carbonic Anhydrase 2 (CA2) catalyzes the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa). CA2 can bind to various transporters, such as anion exchanger 1 (AE1), NBCe1 (Na(+)/HCO3(-) cotransporter), NHE3 (Na(+)/H(+) exchanger 3) and MCT (monocarboxylate transporter). This association increases the rate of transport. Mutations in the CA2 gene are responsible for carbonic anhydrase II (CAII) deficiency syndrome, an autosomal recessive osteopetrosis characterised with renal tubular acidosis as well as cerebral calcifications. Changes in the expression of CA2 are linked with carcinogenesis.

Stato fisico

Lyophilized from 0.22 μm filtered solution in 20 mM Tris, pH 8.0, with 150 mM NaCl, 1 mM DTT. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Ricostituzione

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Pittogrammi

Exclamation mark

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Eye Irrit. 2

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Characterization of CA XIII, a novel member of the carbonic anhydrase isozyme family.
Lehtonen J, et al.
The Journal of Biological Chemistry, 279, 2719-2719 (2004)
Carbonic anhydrase II binds to and increases the activity of the epithelial sodium-proton exchanger, NHE3.
Krishnan D, et al.
American Journal of Physiology: Renal Physiology, 309, F383-F383 (2015)
Carbonic anhydrase 2-like a and 15a are involved in acid-base regulation and Na+ uptake in zebrafish H+-ATPase-rich cells.
Lin TY, et al.
American Journal of Physiology. Cell Physiology, 294, C1250-C1250 (2008)
Amplification of chromosome 8q21-qter associated with the acquired paclitaxel resistance of nasopharyngeal carcinoma cells.
Li W, et al.
International Journal of Clinical and Experimental Pathology, 8, 12346-12346 (2015)
Increased water flux induced by an aquaporin-1/carbonic anhydrase II interaction.
Vilas G, et al.
Molecular Biology of the Cell, 26, 1106-1106 (2015)

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