SRP6472

Transferrin human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Sinonimo/i: DKFZp781D0156, PRO1557, PRO2086, TF
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in HEK 293 cells
• Tag: 6-His tagged (C-terminus)
• Saggio: ≥95% (SDS-PAGE)
• Forma fisica: lyophilized
• PM: calculated mol wt 76 kDa; observed mol wt 74 kDa by SDS-PAGE (DTT-reduced.)
• Confezionamento: pkg of 100 μg; pkg of 20 μg
• Impurezze: <1 EU/μg endotoxin (LAL test)
• N° accesso UniProt: P02787
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... TF(7018)

Descrizione

Descrizione generale

Transferrin is encoded by the TF gene that is mapped to human chromosome 3q22.1. It is a glycoprotein containing homologous iron-binding domains at N-terminal and C-terminal ends. Transferrin has a molecular weight of 80,000 Da and belongs to the transferrin superfamily, the members of which include lactoferrin, melanotransferrin, and ovotransferrin.

Applicazioni

To supplement neurosphere culture medium and sphere differentiation medium.

Azioni biochim/fisiol

Transferrin is an iron-binding glycoprotein that primarily functions in the transport of plasma iron in vertebrates. It may also have a secondary function of facilitating cell proliferation. The binding of ferric iron to transferrin requires the presence of an anion, that functions as a bridge between the metal and the protein.

Stato fisico

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Ricostituzione

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3