SRP3151
SCF human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Sinonimo/i:
Steel Factor, Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF)
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About This Item
Codice UNSPSC:
12352202
NACRES:
NA.32
Prodotti consigliati
Origine biologica
human
Ricombinante
expressed in E. coli
Saggio
≥98% (HPLC)
≥98% (SDS-PAGE)
Forma fisica
lyophilized
Potenza
≤2.0 ng/mL ED50
PM
18.4 kDa
Confezionamento
pkg of 10 μg
tecniche
cell culture | mammalian: suitable
Impurezze
<0.1 EU/μg endotoxin, tested
Colore
white
N° accesso UniProt
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... KITLG(4254)
Descrizione generale
SCF (stem cell factor) is a hematopoietic cytokine that exists in both soluble and transmembrane forms, which arise due to alternative splicing. Both these forms are functionally active. This gene is localized to human chromosome 12q22-12q24. Endothelial cells and fibroblasts constitutively produce both transmembrane and soluble forms of SCF. It is also produced by keratinocytes in normal skin, and epithelial cells in the gut, and thymus. Recombinant human SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.
Azioni biochim/fisiol
SCF (stem cell factor) exerts its activity by signaling through the c-Kit receptor. They participate in hematopoiesis, and development of mast cells and melanocytes. Mutations in either of these proteins result in various phenotypic disorders, such as varying degrees of macrocytic anemia, suppressed numbers of tissue mast cells, reduced fertility, and decreased pigmentation. Linkage analysis along with whole-exome sequencing, c.286_303delinsT (p.Ser96Ter), shows a heterozygous truncating mutation in this gene linked with congenital and stable non-syndromic unilateral and asymmetric hearing loss. rs4590952 polymorphism in this gene is linked with disruption of p53 function through increase in p53-dependent SCF expression, which results in increased susceptibility to testicular cancer.
Sequenza
MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA
Stato fisico
Lyophilized from 10 mM Acetic Acid.
Ricostituzione
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Skin Irrit. 2
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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I clienti hanno visto anche
Stem cell factor and hematopoiesis.
Broudy VC
Blood, 90(4), 1345-1364 (1997)
Allelic Mutations of KITLG, Encoding KIT Ligand, Cause Asymmetric and Unilateral Hearing Loss and Waardenburg Syndrome Type 2.
Zazo Seco C, et al.
American Journal of Human Genetics, 97(5), 647-660 (2015)
A functional p53 responsive polymorphism in KITLG, rs4590952, does not affect the risk of breast cancer.
Chen W, et al.
Scientific Reports, 4:6371 (2014)
T R Ulich et al.
Blood, 78(3), 645-650 (1991-08-01)
Recombinant rat stem cell factor (rrSCF) administered to rats as a single intravenous injection causes a dose-dependent neutrophilia and lymphocytosis as well as the appearance of immature myeloid cells and occasional blast cells in the circulation. Neutrophilia begins at 2
Sibylle A Brenner et al.
Immunology, 143(2), 174-183 (2014-04-05)
Mast cells are now considered sentinels in immunity. Given their location underneath the gastrointestinal barrier, mast cells are entrusted with the task of tolerating commensal microorganisms and eliminating potential pathogens in the gut microbiota. The aim of our study was
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