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Key Documents

SRP0246

Sigma-Aldrich

LKB1/MO25/STRAD Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Sinonimo/i:

STK11, Serine/threonine kinase 11

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About This Item

Codice UNSPSC:
12352204
NACRES:
NA.32

Origine biologica

human

Ricombinante

expressed in baculovirus infected insect cells

Saggio

≥70% (SDS-PAGE)

Forma fisica

aqueous solution

PM

49 kDa (LKB1)
 66 kDa (MO25)
 74 kDa (STRAD)

Confezionamento

pkg of 20 μg

Condizioni di stoccaggio

avoid repeated freeze/thaw cycles

Concentrazione

>0.02 mg/mL

N° accesso NCBI

NM_000455 (STK11)
NM_001003787 (STRAD)
NM_016289 (MO25)

N° accesso UniProt

Q15831

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Informazioni sul gene

human ... STK11(6794)

Descrizione generale

The product is a complex of human Liver kinase B1 (LKB1), also known as serine/threonine kinase 11 (STK11) (GenBank Accession No. NM_000455; amino acids 2-433, with N-terminal His-tag), mouse protein- 25 (MO25) or calcium binding protein 39 (CAB39) (GenBank Accession No. NM_016289; amino acids 2-341, with N-terminal GST-tag) and STE20-related kinase adaptor alpha (STRAD) (GenBank Accession No. NM_001003787, a.a. 2-431, with N-terminal GST-tag).

Applicazioni

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Azioni biochim/fisiol

Liver kinase B1 (LKB1), also known as serine/threonine kinase 11 (STK11), is a serine/threonine kinase that regulates cell polarity and functions as a tumor suppressor protein. LKB1 regulates the activity of adenosine monophosphate-activated protein kinase (AMPK), promoting the suppression of growth and proliferation under energetically unfavorable conditions. Mouse protein-25 (MO25) or calcium binding protein 39 (CAB39) is a scaffolding protein which modulates the activities of STE20 family protein kinases. The MO25a isoform is part of the LKB1 tumor suppressor complex. STE20-related kinase adaptor α (STRAD) is a pseudokinase. It forms a heterotrimeric complex with LKB1 and MO25. The LKB1/MO25/STRAD complex modulates the metabolism, proliferation and cellular polarity of human intestinal epithelial cells.

Stato fisico

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Structural insights into the activation of MST3 by MO25.
Mehellou Y
Biochemical and Biophysical Research Communications, 431(3), 604-609 (2013)
P A Marignani et al.
Cancer biology & therapy, 6(10), 1627-1631 (2007-10-09)
STRADalpha is a pseudokinase that forms a heterotrimeric complex with the scaffolding protein MO25 and the tumor suppressor serine threonine protein kinase LKB1. Mutations in the LKB1 gene are responsible for the Peutz-Jeghers Syndrome (PJS) characterized by a predisposition to
Yue Zong et al.
Cell research, 29(6), 460-473 (2019-04-06)
AMPK, a master regulator of metabolic homeostasis, is activated by both AMP-dependent and AMP-independent mechanisms. The conditions under which these different mechanisms operate, and their biological implications are unclear. Here, we show that, depending on the degree of elevation of
Naomi X Y Ling et al.
Nature metabolism, 2(1), 41-49 (2020-01-30)
Central to cellular metabolism and cell proliferation are highly conserved signalling pathways controlled by mammalian target of rapamycin (mTOR) and AMP-activated protein kinase (AMPK)1,2, dysregulation of which are implicated in pathogenesis of major human diseases such as cancer and type

Articoli

Oncogenes and Tumor Suppressors Reprogram Metabolism

Sigma-Aldrich presents an article about how proliferating cells require the biosynthesis of structural components for biomass production and for genomic replication.

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