SRP0237

PI3 kinase (p120g) Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

• Sinonimo/i: PIK3CG, Phosphoinositide-3-kinase, catalytic, gamma, p120-PI3K
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in baculovirus infected insect cells
• Saggio: ≥90% (SDS-PAGE)
• Stato: aqueous solution
• Attività specifica: ≥1.4 pmol/min-μg
• PM: 131 kDa
• Confezionamento: pkg of 20 μg
• Concentrazione: >0.02 mg/mL
• N° accesso NCBI: AF327656
• N° accesso UniProt: P48736
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... PIK3CG(5294)

Descrizione

Descrizione generale

Human p120γ ( GenBank Accession No. AF327656), full length with N-terminal His tag, MW = 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Applicazioni

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definizione di unità

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Stato fisico

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.