SRP0128
DNMT3a Active human
recombinant, expressed in baculovirus infected insect cells
Sinonimo/i:
DNA (cytosine-5-)-methyltransferase 3A, DNA MTase HsaIIIA, DNMT3A2, M.HsaIIIA
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About This Item
Prodotti consigliati
Origine biologica
human
Ricombinante
expressed in baculovirus infected insect cells
Forma fisica
aqueous solution
PM
128 kDa
Confezionamento
pkg of 10 μg
Concentrazione
>0.02 mg/mL
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
Informazioni sul gene
human ... DNMT3A(1788)
Descrizione generale
DNMT3A (DNA methyltransferase 3α) is one of three (DNMT1, DNMT3a and DNMT3b) active forms of DNA methyltransferases. This gene is localized to human chromosome 2p23. DNMT3A is one of the three members of the DNMT3A family, of which DNMT3A and DNMT3B are catalytically active and DNMT3L is an inactive paralogue, which has regulatory actions in germ cells.
Applicazioni
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Azioni biochim/fisiol
DNA methyltransferases are responsible for the addition of a methyl residue to the cytosine group of CpG dinucleotides. DNMT3A (DNA methyltransferase 3α) participates in the gene expression suppression by interacting with histone modifiers, such as polycomb-group (PcG). The interaction between these two proteins is thought to be implicated in cancer pathogenesis. During embryogenesis this enzyme is responsible for the formation of genomic methylation patterns and germ line development. Polymorphisms in this gene might serve as biomarkers to determine the prognosis of gastric cancer patients. Mutations in this gene are linked with Tatton-Brown-Rahman syndrome, an overgrowth syndrome characterized by unique facial appearance, intellectual disability, and increased height.
Stato fisico
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.
Nota sulla preparazione
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Certificati d'analisi (COA)
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
DNMT3A mutations in acute myeloid leukemia.
The New England Journal of Medicine, 363(25), 2424-2433 (2010)
DNA methyltransferase 3a rs1550117 genetic polymorphism predicts poor survival in gastric cancer patients.
International Journal of Clinical and Experimental Pathology, 8(11), 14864-14874 (2015)
DNMT3A R882 mutants interact with polycomb proteins to block haematopoietic stem and leukaemic cell differentiation.
Nature Communications, 7, 10924-10924 (2016)
Tatton-Brown-Rahman syndrome due to 2p23 microdeletion.
American Journal of Medical Genetics. Part A, 170A(5), 1339-1342 (2016)
Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation.
Nature, 449(7159), 248-251 (2007)
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