SBR00047

Rhodamine B labeled Ramoplanin

For fluorescent microbial imaging

• Formula empirica (notazione di Hill): C₁₄₆H₁₈₄ClN₂₃O₄₆S₂
• Peso molecolare: 3096.73
• Codice UNSPSC: 12352116
• NACRES: NA.76

Proprietà

• Livello qualitativo: 100
• Forma fisica: solid
• Attività specifica: Antibiotic (bacterial staining)
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Rhodamine B labeled Ramoplanin is a fluorescent derivative of Ramoplanin, with a similar mode of action as Ramoplanin. It allows fluorescent labeling of Gram-positive bacteria and their detection by common fluorescent instruments. Ramoplanin is a lipoglycodepsipeptide antibiotic that is produced by the fermentation of Actinoplanes species. Fluorescent antibiotics are obtained by a synthetic conjugation of an antibiotic to a fluorophore.

Applicazioni

Fluorescent antibiotics can be used for many applications including:

• Antimicrobial resistance research
• Bacterial visualization and imaging
• Parent antibiotic mode of action research and new antibiotic discovery
• Toxicity studies
• Diagnosis of bacterial infections and tracking their uptake in vivo

Azioni biochim/fisiol

Ramoplanin blocks the bacterial cell wall biosynthesis by specifically binding to the first sugar of lipid I. N-Acetylmuramic acid thus interfering with peptidoglycan production.

Mode of Action: Is different from the d-Ala-d-Ala that are targeted by vancomycin and shows no cross-resistance with other glycopeptides.

Activity Spectrum: Effective against a broad-spectrum activity against Gram-positive pathogens both in vitro and in vivo including Enterococci, Staphylococci, Bacilli, Streptococci, Listeria monocytogenes, and Gram-positive anaerobes such as Clostridrium difficile. However, Rhodamine B labeled Ramoplanin presents a MIC decrease in the magnitude of two to five orders in bacterial activity in comparison to the parent Ramoplanin.

Caratteristiche e vantaggi

High quality antibiotic suitable for mulitple research applications

Risultati analitici

• Fluorescent microscopy application: Rhodamine B labeled Ramoplanin has excitation/emission wavelength range at 550-560 nm/590-600 nm.
• It is recommended to dissolve the Rhodamine B labeled Ramoplanin in DMSO to a concentration of 2 mg/mL.
• The recommended working concentration in fluorescent microscopy imaging application is 10μM. The mentioned concentration was used for Bacillus subtills staining (see image).
• Aliquots of the DMSO solution can be stored at −20 °C, protected from light for at least one month.

Altre note

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Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable