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Key Documents

SBR00029

Sigma-Aldrich

Dansyl labeled polymyxin B Ready Made Solution

for fluorescent microbial imaging, 1.5 mg/mL in H2O

Sinonimo/i:

DSL-PMB, DSL-PMX, DSL-PxB

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About This Item

Codice UNSPSC:
51102829
NACRES:
NA.76

Livello qualitativo

100

Forma fisica

solution

Disponibilità

not available in China

Concentrazione

1.5 mg/mL in H2O

Colore

colorless to light brown

Compatibilità

suitable for (Fluorescent detection of gram-negative bacteria)
suitable for (Microbiome)
suitable for microbiology

Modalità d’azione

cell membrane

Condizioni di spedizione

ambient

Temperatura di conservazione

−20°C

Descrizione generale

Dansyl Polymyxin B (DSL-PMB) is a fluorescent derivative of Polymyxin B. Polymyxin B is a cationic lipopeptide antibiotic that binds specifically to Gram-negative bacteria. Fluorescent antibiotics are obtained by synthetic conjugation of an antibiotic to a fluorophore.
Dansyl labeled Polymyxin B can be utilized for fluorescent microbial imaging in the fields of Cell Biology and Biochemical research.

Applicazioni

DSL-PMB may have been used:
  • as a fluorescent probe to study polymyxin mode of action and its pharmacokinetics
  • in research and to develop new active derivatives of Polymyxin against multi-drug resistance Gram-negative infections
  • to measure LPS binding affinity by fluorometric displacement assay
Fluorescent antibiotics can be used for many applications including:
  • Antimicrobial resistance research.
  • Bacterial visualization and imaging.
  • Parent antibiotic mode of action research and new antibiotic discovery.
  • Toxicity studies.
  • Research of bacterial infections and tracking its uptake in vivo.

Azioni biochim/fisiol

DSL-PMB′s mode of action is in accordance with Polymyxin B activity. However, MIC assays comparing DSL-PMB, and parent Polymyxin B showed a two to three-fold decrease in bacterial activity. Polymyxin B interacts with anionic lipopolysaccharide (LPS) molecules which are located in Gram-negative bacterial outer membrane. This interaction interferes with the outer membrane′s normal function, causing leakage and enhanced uptake of the antibiotic. The resemblance in properties between Polymyxin B and DSL-PMB allows to fluorescently label Gram-negative bacteria and examine intracellular localization and penetration of Polymyxins in Gram-negative bacteria.

Caratteristiche e vantaggi

  • High-quality antibiotic suitable for multiple research applications
  • Ideal for Cell Biology, Metabolomics, and Biochemical research.

Risultati analitici

  • Dansyl Labeled Polymyxin B Ready Made Solution is light sensitive.
  • It is recommended to avoid freeze-thaw cycles of PMB-DSL Ready Made Solution.
  • Dansyl Labeled Polymyxin B Ready Made Solution (1.5 mg/mL) can be diluted 1:50 in PBSX1 (Sigma#D8537) to achieve 30 μg/mL final concentration for staining. The above concentration of DSL-PMB was used for E. coli staining see image.
  • Fluorescence Microscopy application: Dansyl Labeled Polymyxin B Ready Made Solution excitation (Ex) wavelength is 330-340nm resulting in emission (Em) range of 540-600nm (λmax=570nm).

Altre note

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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R A Moore et al.
Antimicrobial agents and chemotherapy, 30(6), 923-926 (1986-12-01)
Pseudomonas cepacia was found to be resistant to the outer membrane-permeabilizing effects of aminoglycoside antibiotics, polymyxin B, and EDTA. Permeabilization of P. cepacia to the fluorescent probe 1-N-phenylnaphthylamine was not achieved at concentrations 100- to 1,000-fold above those required to
P R Schindler et al.
Antimicrobial agents and chemotherapy, 8(1), 95-104 (1975-07-01)
Though the primary action of the cationic antibiotic polymyxin B is against the membrane of susceptible bacteria, severe morphological changes are detected in the cytoplasm. Using fluorescence microscopy and a mono-N-dansyl-polymyxin B derivative, we could demonstrate aggregations of the antibiotic
Vincent H Tam et al.
Antimicrobial agents and chemotherapy, 49(9), 3624-3630 (2005-08-30)
Despite limited data, polymyxin B (PB) is increasingly used clinically as the last therapeutic option for multidrug-resistant (MDR) gram-negative bacterial infections. We examined the in vitro pharmacodynamics of PB against four strains of Pseudomonas aeruginosa. Clonal relatedness of the strains
R E Hancock
Lancet (London, England), 349(9049), 418-422 (1997-02-08)
The era of the "classical antibiotic" may be over. The emergence of resistance has seen to that. Yet no truly novel class of antibacterial agent has come on the market in the past 30 years. Currently there is great interest
M E Evans et al.
The Annals of pharmacotherapy, 33(9), 960-967 (1999-09-24)
Polymyxin B sulfate and colistin, also known as colistimethate, have not been used for many years because less toxic antimicrobials are available. Gram-negative bacteria that are resistant to the aminoglycosides, beta-lactams, and fluoroquinolones are becoming more common. These bacteria are

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