SAE0156
Bone Alkaline Phosphatase from human bone osteosarcoma cells
lyophilized, ≥2000 u/mg protein
Sinonimo/i:
ALPL, BAP, Bone ALP, Ostase
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12352204
NACRES:
NA.77
Prodotti consigliati
Descrizione generale
Mammalian alkaline phosphatases are a group of membrane-bound glycoprotein isoenzymes that are attached to the cell membrane by a hydrophobic glycosyl-phosphatidylinositol (GPI) anchor.
The bone alkaline phosphatase isozyme is expressed from the gene for tissue nonspecific alkaline phosphatase which is also expressed in liver and kidney. These three isoforms form the majority of alkaline phosphatases circulating in serum. All three forms share the same amino acid sequence but have different carbohydrate and lipid modifications that provide unique properties according to the source tissue.
Bone alkaline phosphatase is synthesized by osteoblasts, involved in the calcification of bone matrix, but its precise role in bone formation process is still unknown. It is a highly specific marker of the bone-forming activity of osteoblasts. High activity of bone alkaline phosphatase is observed in serum in several bone diseases, such as Paget′s disease, osteoporosis, ricket disease, bone metastatic carcinoma, and others. Physiological bone growth can also contribute to increased levels of bone alkaline phosphatase in serum.
This product is purified from human osteosarcoma Saos-2 cells, an established cell line with high basal alkaline phosphatase activity. Before purification, alkaline phosphatase is released from the cell membrane by enzymatic cleavage of the GPI anchor.
The bone alkaline phosphatase isozyme is expressed from the gene for tissue nonspecific alkaline phosphatase which is also expressed in liver and kidney. These three isoforms form the majority of alkaline phosphatases circulating in serum. All three forms share the same amino acid sequence but have different carbohydrate and lipid modifications that provide unique properties according to the source tissue.
Bone alkaline phosphatase is synthesized by osteoblasts, involved in the calcification of bone matrix, but its precise role in bone formation process is still unknown. It is a highly specific marker of the bone-forming activity of osteoblasts. High activity of bone alkaline phosphatase is observed in serum in several bone diseases, such as Paget′s disease, osteoporosis, ricket disease, bone metastatic carcinoma, and others. Physiological bone growth can also contribute to increased levels of bone alkaline phosphatase in serum.
This product is purified from human osteosarcoma Saos-2 cells, an established cell line with high basal alkaline phosphatase activity. Before purification, alkaline phosphatase is released from the cell membrane by enzymatic cleavage of the GPI anchor.
Definizione di unità
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate to p-nitrophenol and inorganic phosphate per minute at pH 9.8 at 37 °C.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.