SAE0045

HRV-3C Protease.

N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL

• Sinonimo/i: Human Rhinovirus 3C Protease, Levlfqgp site protease, PreScission Protease
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Origine biologica: human (human Rhinovirus Type 14)
• Livello qualitativo: 200
• Saggio: ≥90% (SDS-PAGE)
• Forma fisica: aqueous solution
• Attività specifica: ≥5000 U/mg
• PM: 21 kDa
• Concentrazione: 0.8-1.2 mg/mL
• tecniche: protein purification: suitable
• Compatibilità: suitable for protein modification
• applicazioni: life science and biopharma
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

HRV-3C protease from human rhinovirus type 14 is a protease that specifically cleaves within an eight-residue recognition sequence.
Proteolytic cleavage occurs between the Gln and Gly residues.
HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest.
This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography.

Applicazioni

HRV-3C Protease has been used to remove the GST tag from the DsCystatin protein.

Definizione di unità

One unit of HRV-3C Protease is defined as the amount of enzyme needed to digest 1nmole of the substrate peptide per hour (H-Glu-Ala-Leu-Phe-Gln-pNA) at 0 °C, in a reaction buffer consisting of 25 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.

Stato fisico

Supplied in a solution containing 50 mM Tris HCl (pH 7.5), 0.15 M NaCl, 1 mM TCEP and 50% (V/V) glycerol.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2