SAB5801018

Alpaca Anti-Mouse IgM(µ chain) single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• Sinonimo/i: Recombinant Alpaca single variable domain secondary Nanobody
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: alpaca
• Ricombinante: expressed in E. coli
• Coniugato: unconjugated (6*his tag and one cys were added at the C terminal of the VHH)
• Forma dell’anticorpo: affinity purified immunoglobulin
• Tipo di anticorpo: secondary antibodies
• Clone: recombinant monoclonal
• Forma fisica: lyophilized solid
• PM: 14 kDa
• Reattività contro le specie: Alpaca, mouse
• tecniche: ELISA: 1:5000-1:20000; western blot: 1:5000-1:20000
• Misura: 0.1 mL
• Colore: colorless
• Isotipo: VHH
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Alpaca Anti-Mouse IgM(µ chain) single domain Antibody is designed to specifically detect mouse IgM. It is a monovalent, single domain, recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse IgM(µ chain) single domain Antibody reacts with the µ chain of mouse IgM selectively but not with mouse, rabbit, human, cynomolgus, rat, goat IgG.

Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. IgM accounts for 5-10% of the immunoglobulin pool and is the predominant antibody in the primary immune response. Unlike IgG, IgM does not contain a hinge region but does contain an additional constant domain. The monomeric form IgM has a molecular weight of 180 KD. It is classically represented as a pentamer of the basic four chain structure held together by a J chain but can also exist in a hexameric form without the J chain and as a monomer on the surface of B-cells.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Specificità

Fc region of mouse IgM (µ chain specific)
Isotype: VHH domain of alpaca IgG2b/2c

Immunogeno

Recombinant Alpaca single variable domain secondary nano antibody

Confezionamento

100 μg in Glass bottle

Stato fisico

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Ricostituzione

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Stoccaggio e stabilità

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

Altre note

No cross-reactivity with mouse, rabbit, human, cynomolgus, rat, goat IgG

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H317,H412
• Consigli di prudenza: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Classi di pericolo: Aquatic Chronic 3 - Skin Sens. 1
• Codice della classe di stoccaggio: 13 - Non Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable