SAB4200821
Anti-Fibroblast Surface Protein antibody, Mouse monoclonal
clone 1B10, purified from hybridoma cell culture
Sinonimo/i:
FSP
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12352203
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Forma dell’anticorpo
purified from hybridoma cell culture
Tipo di anticorpo
primary antibodies
Clone
1B10, monoclonal
Reattività contro le specie
human
Confezionamento
antibody small pack of 25 μL
Concentrazione
~ 1 mg/mL
tecniche
immunofluorescence: 5-10 μg/mL using Human foreskin fibroblast Hs68 cells.
Isotipo
IgM
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... S100A4(6275)
Descrizione generale
A surface protein characterizing human fibroblasts and fibroblast cell lines, as well as tissue macrophages and 95% of the peripheral blood monocytes. This fibroblast protein antigen has also been demonstrated on malignant fibrosarcoma tissue, human synovial, foreskin and thymic fibroblasts and it is absent from human epithelial cells and lymphocytes.
Specificità
Monoclonal Anti-Human Fibroblast Surface Protein specifically recognizes human fibroblast cells, tissue macrophages and peripheral monocytes .Due to its specific reaction against human fibroblasts, the antibody is useful for removal of fibroblasts from human cultured cells either by inhibition of fibroblasts adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement.
Immunogeno
Cultured human thymic fibroblasts
Applicazioni
The antibody is recommended for use in various immunological techniques including Immunofluorescence, flow cytometry, immunohistochemistry and immunoblotting.
Azioni biochim/fisiol
Overgrowth of epithelial cells by fibroblasts has long been an obstacle to cultivation of normal and abnormal epithelial cells.Characterization and identification of cells is important for studying functional significance, correlation of cultures with tissue origin, identification of the lineage to which the cells belongs to or identifying the precursor status of the cells.
Stato fisico
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Stoccaggio e stabilità
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Esclusione di responsabilità
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Non trovi il prodotto giusto?
Prova il nostro Motore di ricerca dei prodotti.
Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
C N Abboud et al.
Blood, 68(6), 1196-1200 (1986-12-01)
The murine IgG2a monoclonal antibody 6-19 binds to a wide variety of nonhematopoietic cells including human marrow-derived stromal cells but does not bind to marrow or peripheral blood cells. We studied the effects of this antibody and rabbit complement on
K H Singer et al.
Human immunology, 13(3), 161-176 (1985-07-01)
Long-term in vitro cultures of human thymic tissue were established and phenotypically characterized using monoclonal reagents that define distinct components of the human thymic microenvironment. The epithelial component of the thymus, defined by monoclonal antibodies TE-3, TE-4, BBTECS, and AE1
N SundarRaj et al.
Current eye research, 3(4), 637-644 (1984-04-01)
Several monoclonal antibodies were developed to cell-surface antigens of human corneal fibroblasts grown in culture. Indirect immunohistochemical analysis showed that the antigens recognized by these monoclonal antibodies were also present on the skin fibroblasts. However, these antigens were not detectable
K H Singer et al.
The Journal of investigative dermatology, 92(2), 166-170 (1989-02-01)
A complement fixing IgM monoclonal antibody (1B10) that reacts with surface membrane molecules of human fibroblasts, tissue macrophages, and peripheral monocytes was produced. In Western blot analysis of detergent extracts of cultured human foreskin fibroblasts, antibody 1B10 detected protein bands
Lei Shangguan et al.
Stem cells (Dayton, Ohio), 30(12), 2810-2819 (2012-10-05)
Bone marrow mesenchymal stem cells (BM-MSCs) have multiple therapeutic potentials for regenerative, anti-inflammatory, and immunomodulatory purposes and also show promise as vehicles for gene therapy of various metastatic cancers based on their tumor-tropic capacity. However, BM-MSCs are also a source
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.