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Key Documents

SAB4200741

Sigma-Aldrich

Anti-Human IgE antibody, Mouse monoclonal

clone GE-1, purified from hybridoma cell culture

Sinonimo/i:

Anti-Immunoglubulin E

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified from hybridoma cell culture

Tipo di anticorpo

primary antibodies

Clone

GE-1, monoclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

human

Concentrazione

~1.0 mg/mL

tecniche

ELISA: 0.15-0.3 μg/mL using 1 μg/mL Human IgE myeloma for coating
immunoblotting: suitable
immunoprecipitation (IP): 1-2 μg/test using Human IgE myeloma

Isotipo

IgG2b

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Anti-Human IgE antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the GE-1 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified human IgE myeloma protein. Immunoglobulin E (IgE) is the least abundant immunoglobulin isotype in circulation. IgE is produced by plasma cells and is highly functional in the lung, skin and mucus membranes. Normal levels of IgE in serum are less than 1 μg/ml.

Immunogeno

purified human IgE myeloma protein

Applicazioni

Anti-Human IgE antibody, Mouse monoclonal has been used in various immunological techniques, including enzyme linked immunosorbent assay (ELISA), immunoblot, immunoprecipitation and IgE quantification using piezoelectric (Pz) crystal bioassay.

Azioni biochim/fisiol

Immunoglobulin E (IgE) plays a critical role in allergic reactions and host protection from parasites. Serum IgE levels are highly elevated during an allergic reaction (such as atopic eczema, extrinsic asthma or hay fever, fungal and parasitic infection, atopic disease or primary immunodeficiency). Detection and measurement of total IgE concentrations is crucial for diagnosis, monitoring and treatment of allergy patients. In addition, the recognition and isolation of allergen-specific IgE can lead to progress in understanding of pathophysiologic mechanisms underlying IgE-associated allergic disorders.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Immunoglobulin E in health and disease
Amarasekera M, et al.
Asia Pacific Allergy, 1(1), 12-15 (2011)
Allergic and nonallergic forms of atopic diseases
Novak N and Bieber T
The Journal of Allergy and Clinical Immunology, 112(2), 252-262 (2003)
Precision medicine allergy immunoassay methods for assessing immunoglobulin E sensitization to aeroallergen molecules
Popescu FD, et al.
World journal of methodology, 8, 17-17 (2018)
Factors affecting human IgE and IgG responses to allergen-like Schistosoma mansoni antigens: Molecular structure and patterns of in vivo exposure
Fitzsimmons CM, et al.
International Archives of Allergy and Immunology, 142, 40-50 (2019)
R H Buckley et al.
The Journal of clinical investigation, 55(1), 157-165 (1975-01-01)
Concentrations of IgD and IgE were measured in sera from 165 patients with well-defined immunodeficiency in an effort to find information possibly relevant to the roles of antibodies of these classes in host defense. Values for both immunoglobulins were generally

Articoli

Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.

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