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Key Documents

S2454

Millipore

Seppro Ammonium Bicarbonate Buffer

Sinonimo/i:

ABC buffer, Ammonium hydrogen carbonate, Monoammonium carbonate

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.32

Forma fisica

solution

Livello qualitativo

Concentrazione

2 M

tecniche

protein expression: suitable

Colore

clear colorless

applicazioni

life science and biopharma

Temperatura di conservazione

room temp

Descrizione generale

Seppro® Ammonium Bicarbonate Buffer is an effective buffer for protein digestion and deglycosylation reaction. It may be used to coat 2D and 3D electrophoretic gel pieces during digestion of proteins and proteomes. The ammonium bicarbonate buffer also provides moisture during enzymatic cleavage. Ammonium bicarbonate buffer regulates the microenvironmental pH of immobilized cephalosporin C acylase (CCA) for the catalysis of cephalosporin C (CPC) in a batch stirred tank reactor (STR).

Applicazioni

Ammonium bicarbonate buffer has been used as a component of cell lysis/protein extraction buffer for proteomic sample preparation.

Note legali

6538 is a trademark of American Type Culture Collection
Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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The Journal of biological chemistry, 278(36), 34189-34202 (2003-06-12)
The zona pellucida is an extracellular matrix consisting of three glycoproteins that surrounds mammalian eggs and mediates fertilization. The primary structures of mouse ZP1, ZP2, and ZP3 have been deduced from cDNA. Each has a predicted signal peptide and a
Andrej Shevchenko et al.
Nature protocols, 1(6), 2856-2860 (2007-04-05)
In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel
Vimal K Balasubramanian et al.
Current protocols, 1(5), e153-e153 (2021-05-28)
Plant organs and tissues contain multiple cell types, which are well organized in 3-dimensional structure to efficiently perform physiological functions such as homeostasis and response to environmental perturbation and pathogen infection. It is critically important to perform molecular measurements at

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