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P6611

Millipore

HIS-Select® Nickel Affinity Gel

(1:1 suspension in a 20% ethanol solution)

Sinonimo/i:

Ni-NTA resin, nickel charged agarose

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.56

Coniugato

magnetic beads

Livello qualitativo

200

Stato

(1:1 suspension in a 20% ethanol solution)

Caratteristiche

hydrophilic

Confezionamento

pkg of 1 mL
pkg of 100 mL
pkg of 25 mL
pkg of 5 mL
pkg of 500 mL

Concentrazione

1.5-2.4 mL/mL (suspension in packed gel)

tecniche

protein purification: suitable

Colore

faint blue to very dark blue

Matrice

6% Beaded Agarose

Capacità

>15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

Temp. transizione

flash point 32 °C (closed cup)

Temperatura di conservazione

2-8°C

Categorie correlate

Descrizione generale

HIS-Select® Nickel Affinity Gel is an immobilized metal ion affinity chromatography (IMAC) product, used for the purification of His-tagged proteins. While the unique, non-charged, hydrophilic linkage of the proprietary quadridentate NTA chelate group to the beaded agarose charged with nickel ensures high selectivity for small to medium scale His-tag protein purification, it also results in reduced non-specific binding of other proteins. HIS-Select Nickel Affinity Gel is selective for recombinant proteins with His-tags and exhibits low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography.

Applicazioni

HIS-Select® Nickel Affinity Gel has been used in the purification of recombinant proteins like EF-hand calcium-binding protein (S100A14), LIM homeobox transcription factor 1 alpha protein, sigma-1 receptor as well as harpin, stable protein 1 (SP1), and BCR-ABL fusion protein.

Caratteristiche e vantaggi

  • High selectivity for higher purity.
  • Unique non-charged hydrophilic linkage reduces non-specific binding.
  • Binding capacity for histidine-tagged protein is greater than 15 mg/mL.
  • Binding under denaturing or non-denaturing conditions.
  • One-step purification.
  • Minimizes unwanted ionic interactions.
  • Minimal nickel leaching.
  • Bead size: 45-165 μm.

Linkage

It is also available with the EZview™ technology (Product Code E3528).

Stato fisico

1:1 suspension in a 20% ethanol solution

Stoccaggio e stabilità

HIS-Select Nickel Affinity Gel is stable for at least one year when stored properly. The HIS-Select Nickel Affinity Gel should be cleaned after each use and an antimicrobial agent such as 20% ethanol should be added to the storage buffer.

Note legali

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

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Pittogrammi

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Avvertenze

Warning

Indicazioni di pericolo

H226

Classi di pericolo

Flam. Liq. 3

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

89.6 °F - closed cup

Punto d’infiammabilità (°C)

32 °C - closed cup

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
SLCK4359
SLBX5337
SLBV4634
SLBS6881
SLBQ9871V

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