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Key Documents

P3804

Sigma-Aldrich

′o-fenilendiammina

chromogenic, tablet

Sinonimo/i:

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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About This Item

Formula condensata:
C6H4(NH2)2·2HCl
Numero CAS:
Peso molecolare:
181.06
Beilstein:
3912045
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
ID PubChem:
NACRES:
NA.83

product name

′o-fenilendiammina, tablet, 5 mg substrate per tablet

Livello qualitativo

Forma fisica

tablet

Punto di fusione

258 °C (dec.) (lit.)

Solubilità

water: 1 tablet/10 mL, clear, colorless

Temperatura di conservazione

2-8°C

Stringa SMILE

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
RIIWUGSYXOBDMC-UHFFFAOYSA-N

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Descrizione generale

Il cloridrato di o-fenilendiammina (OPD) è un substrato cromogeno adatto all'uso nel dosaggio con immunoadsorbente legato a un enzima (ELISA) in quanto si avvale di coniugati a perossidasi di rafano Il substrato sviluppa un prodotto finale solubile arancio-bruno e può essere letto con spettrofotometria a 450 nm. La reazione dell′OPD può essere arrestata con HCl 3N o H2SO4 3 M e può essere letta a 492 nm.

Applicazioni

o-Phenylenediamine dihydrochloride has been used:
  • as a chromogenic substrate in indirect enzyme-linked immunosorbent assay (ELISA) to detect immunoglobin G (IgG) antibodies against certain recombinant antigens/proteins
  • as a streptavidin-horseradish peroxidase (HRP) substrate sandwich ELISA to analyze human interleukin (IL)-17A, IL-23, and IP-10
  • as a substrate in ELISA for determination of IgG-class antibodies to Mycobacterium bovis in serum

Confezionamento

16 mg total tablet weight

Ricostituzione

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

Avvertenze

Warning

Classi di pericolo

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3


Certificati d'analisi (COA)

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Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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