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MTOX1304

Sigma-Aldrich

Canine MDR1 Knockout, Human BCRP Knockin MDCKII Cells

canine kidney (cocker spaniel)

Sinonimo/i:

MDCKII MDR1 KO hBCRP knockin

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.81

Nome del prodotto

Canine MDR1 Knockout, Human BCRP Knockin MDCKII Cells,

Origine biologica

canine kidney (cocker spaniel)

Livello qualitativo

200

Stato

liquid

Temperatura di conservazione

−196°C

Informazioni sul gene

human ... ABCG2(9429)

Applicazioni

See technical bulletin for detailed protocols

Caratteristiche e vantaggi

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells.
The canine MDR1 (cP-gp) efflux transporter gene has been effectively disrupted in both alleles. There is no expression of the cP-gp. Human BCRP has been transfected into the MDCKII cell line. Validation studies have shown efflux of standard BCRP substrates.

Qualità

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Note legali

Exhibit 1: ADME/Tox cell lines license

MDCKII subclone was originally isolated by Daniel Louvard, Institut Curie, Paris France.

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
SLBS0435V

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G C Hansson et al.
The EMBO journal, 5(3), 483-489 (1986-03-01)
The glycosphingolipids (GSLs) of two sublines of Madin-Darby canine kidney (MDCK) cells, an epithelial cell line, were characterized by t.l.c., antibody overlay and mass spectrometry. The major characteristic which distinguishes the two MDCK cell strains is their trans-epithelial electrical resistance
Dominik Gartzke et al.
Pharmaceutical research, 32(6), 2060-2071 (2014-12-20)
To investigate whether it is possible to specifically suppress the expression and function of endogenous canine P-glycoprotein (cPgp) in Madin-Darby canine kidney type II cells (MDCKII) transfected with hPGP and breast cancer resistance protein (hBCRP) by zinc finger nuclease (ZFN)

Articoli

CRISPR Engineered MDCKII Cells Without Canine P-glycoprotein

Madin-Darby Canine Kidney (MDCK) cells are one of the most widely used cell lines for a variety of research applications, including drug permeability and transporter studies.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.