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MP0025

Sigma-Aldrich

Venor™ GeM Mycoplasma Detection Kit, PCR-based

Sinonimo/i:

PCR mycoplasma detection kit

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.75

impiego

 kit sufficient for 25 tests

Livello qualitativo

200

Confezionamento

pkg of 1 kit

Condizioni di stoccaggio

dry at room temperature

tecniche

PCR: suitable

applicazioni

detection
microbiology

Temperatura di conservazione

2-8°C

Categorie correlate

Descrizione generale

The Venor™ GeM Mycoplasma Detection Kit utilizes the polymerase chain reaction (PCR), which was established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals. Detection requires as little as 1–5fg of mycoplasma DNA corresponding to 2–5 mycoplasma per sample volume. The primer set is specific to the highly conserved rRNA operon, or more specifically, the 16S rRNA coding region in the mycoplasma genome. This allows for detection of all Mycoplasma, Acholeplasma and Ureaplasma species tested so far, which are usually encountered as contaminants in cell cultures. Eukaryotic and bacterial DNA are not amplified by this kit.

Applicazioni

The PCR-based Venor™ GeM Mycoplasma Detection Kit employs PCR technology for rapid and reliable detection of mycoplasma DNA in cell cultures and virus stocks.

Componenti

Does not include Taq Polymerase. Optimized for use with D9307, Taq DNA Polymerase

Stoccaggio e stabilità

The kit components are stable during shipping at ambient temperature. Upon receipt, store at 2–8 °C. After rehydration of the primer/nucleotide mix, the positive control, and the internal control, store below –20 °C and avoid repeated freezing and thawing. For repeated testing of low sample numbers, primer/nucleotide mix and controls should be aliquoted after rehydration. By following these recommendations, the kit is stable until the expiration date stated on the label.

Note legali

Venor is a trademark of Minerva Biolabs GmbH

Solo come componenti del kit

N° Catalogo
Descrizione
  • Positive Control
  • Negative Control
  • PCR 10X Reaction Buffer 500 mL/vial
  • Primer/Nucleotide Mix 1 mL/vial

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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