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MIDI67

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Midi Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

Sinonimo/i:

Green PKH membrane midi labeling kit

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.32

Confezionamento

pkg of 1 kit

Livello qualitativo

100

Produttore/marchio commerciale

Distributed for Phanos Technologies

Condizioni di stoccaggio

protect from light

tecniche

flow cytometry: suitable

Fluorescenza

λex 490 nm; λem 502 nm (PKH67 dye)

applicazioni

cell analysis
detection

Metodo di rivelazione

fluorometric

Condizioni di spedizione

ambient

Temperatura di conservazione

room temp

Categorie correlate

Descrizione generale

This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Applicazioni

PKH67 Green Fluorescent Cell Linker Midi Kit has been used for:
  • labeling exosomes  
  • extracellular vesicles (EVs)l
  • normal and sickle red blood cells (RBCs)
  • Raji cells

Linkage

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Note legali

CellVue is a registered trademark of Phanos Technologies

Solo come componenti del kit

N° Catalogo
Descrizione
  • PKH67 Dye 2 x 0.1
  • Diluent C 6 x 10

Pittogrammi

FlameExclamation mark
GHS02,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H225,H319

Classi di pericolo

Eye Irrit. 2 - Flam. Liq. 2

Codice della classe di stoccaggio

3 - Flammable liquids

Punto d’infiammabilità (°F)

57.2 °F - closed cup

Punto d’infiammabilità (°C)

14 °C - closed cup

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
MKCQ8263
MKCP3716
MKCL5579
MKCK8898
MKCK5701

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Dongxin Tang et al.
Clinical and translational medicine, 10(4), e139-e139 (2020-09-09)
Cancer stem cells (CSCs) are important factors contributing to tumorigenesis. We examined whether CSCs isolated from colorectal cancer (CRC) cells possess metastatic properties that can be transferred to non-CSCs via the delivery of miR-200c enclosed in extracellular vesicles (EVs). The
Notochordal-cell derived extracellular vesicles exert regenerative effects on canine and human nucleus pulposus cells
Bach F, et al.
Testing, 8(51), 88845-88845 (2017)
Modulation of Sickle Red Blood Cell Adhesion and its Associated Changes in Biomarkers by Sulfated Nonanticoagulant Heparin Derivative
Alshaiban A, et al.
Clinical and Applied Thrombosis/Hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 22, 230-238 (2016)
ADCC Assay Protocol
Fang L and He F
Journal of Virology (2013)
Chuang Gao et al.
Aging, 11(24), 12147-12164 (2019-12-17)
Exosomes are small (30-150 nm diameter) lipid bilayer-enclosed vesicles found in all bodily fluids. We investigated whether exosomes play a role in chronic subdural hematoma (CSDH). Exosomes were identified and characterized using transmission electron microscopy and NanoSight particle tracking. The
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