Passa al contenuto
Merck
Tutte le immagini(1)

Key Documents

View All Documentation

MINI67

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

Sinonimo/i:

Green PKH membrane mini labeling kit

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(1)

About This Item

Codice UNSPSC:
12352207
NACRES:
NA.32

Confezionamento

pkg of 1 kit

Livello qualitativo

200

Produttore/marchio commerciale

Distributed for Phanos Technologies

Condizioni di stoccaggio

protect from light

tecniche

flow cytometry: suitable

Fluorescenza

λex 490 nm; λem 502 nm (PKH67 dye)

applicazioni

cell analysis
detection

Metodo di rivelazione

fluorometric

Condizioni di spedizione

ambient

Temperatura di conservazione

room temp

Descrizione generale

PKH67 kit is for general cell membrane labeling. It has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Applicazioni

PKH67 green fluorescent cell linker mini kit for general cell membrane labeling has been used to stain:
  • exosomes
  • pericytes
  • UT-7/TPO cells

This kit is for general cell membrane labeling. Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes.

Linkage

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Note legali

Distributed for Phanos Technologies, Inc.
CellVue is a registered trademark of Phanos Technologies

Solo come componenti del kit

N° Catalogo
Descrizione
  • Diluent C 10 mL
  • PKH67 Cell Linker in ethanol 0.1 mL

Pittogrammi

FlameExclamation mark
GHS02,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H225,H319

Classi di pericolo

Eye Irrit. 2 - Flam. Liq. 2

Codice della classe di stoccaggio

3 - Flammable liquids

Punto d’infiammabilità (°F)

55.4 °F - closed cup

Punto d’infiammabilità (°C)

13 °C - closed cup

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Slide 1 of 4

1 of 4

Saccarosio ≥99.5% (GC), BioReagent, suitable for cell culture, suitable for insect cell culture

Sigma-Aldrich

S1888

Saccarosio

Sigma-Aldrich

Sigma-Aldrich

PCLDIL

Diluent B for Phagocytic cell labeling

GW4869 ≥90% (NMR)

Sigma-Aldrich

D1692

GW4869

LuminiCell Tracker 540- Cell Labeling Kit Biocompatible organic fluorescent nanoparticles (AIEDots) that can be used to label cells for long term cell tracking and cell tracing in vitro and in vivo experiments.

Sigma-Aldrich

SCT010M

LuminiCell Tracker 540- Cell Labeling Kit

Exosomal MALAT1 derived from oxidized low-density lipoprotein-treated endothelial cells promotes M2 macrophage polarization
Huang C, et al.
Molecular Medicine Reports, 18(1), 509-515 (2018)
A microfluidic study of megakaryocytes membrane transport properties to water and dimethyl sulfoxide at suprazero and subzero temperatures
Tseng HY, et al.
Biopreservation and Biobanking, 9(4), 355-362 (2011)
Yuan Yang et al.
Experimental and therapeutic medicine, 20(6), 257-257 (2020-11-18)
Mesenchymal stem cell (MSC) infusion has become a novel therapeutic strategy for complex autoimmune diseases; however, few detailed studies have been performed to investigate the benefit and mechanism of MSC treatment on systemic sclerosis (SSc). The present study aimed to
Simone Moertl et al.
International journal of molecular sciences, 21(7) (2020-04-02)
Normal tissue toxicity is a dose-limiting factor in radiation therapy. Therefore, a detailed understanding of the normal tissue response to radiation is necessary to predict the risk of normal tissue toxicity and to development strategies for tissue protection. One component
Hongbin Wang et al.
Molecular therapy. Nucleic acids, 19, 654-667 (2020-01-20)
Recently, novel mechanisms underlying the pro-tumorigenic effects of cancer-associated fibroblasts (CAFs) have been identified in several cancers, including breast cancer. CAFs can secrete exosomes that are loaded with proteins, lipids, and RNAs to affect tumor microenvironment. Herein, we identify CAF-derived
Visualizza tutti i documenti correlati

Articoli

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio. Click here to find protocol for PKH cell labeling

Lipophilic Dye Kits for Cell Tracking

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

Cell Tracking with Lipophilic Membrane Dyes

Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

A video about how you can use fluorescent cell tracking dyes in combination with flow and image cytometry to study interactions and fates of different cell types in vitro and in vivo.

Vedi tutto

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.