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MDQ1

Sigma-Aldrich

Imprint® Methylated DNA Quantification Kit

To measure global DNA methylation shifts from as low as 10 ng DNA

Sinonimo/i:

DNA Methylation Quantification, DNA methylation shift

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About This Item

Codice UNSPSC:
41116158
NACRES:
NA.54

Descrizione generale

Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.

Applicazioni

Imprint® Methylated DNA Quantification Kit has been used in global DNA methylation analysis.

Caratteristiche e vantaggi

  • ELISA based format
  • Procedure completes in 4 easy to follow steps
  • All reagents supplied including control methylated DNA
  • Detection limit is 5 ng of fully methylated DNA
  • Input DNA may be as low as 10-200 ng
  • Procedure completes in 3.5 hours
  • 96 well format allows option of studying single samples or high-throughput studies
  • Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
  • May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum

Note legali

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • M8570Methylated Control DNA, 50 ng/μLSDS

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Pittogrammi

CorrosionExclamation mark
GHS05,GHS07

Avvertenze

Warning

Indicazioni di pericolo

H290,H317,H412

Classi di pericolo

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

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Certificati d'analisi (COA)

Lot/Batch Number

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Kazuhisa Nakano et al.
Journal of immunology (Baltimore, Md. : 1950), 190(3), 1297-1303 (2013-01-02)
Rheumatoid arthritis (RA) is a chronic inflammatory disease in which fibroblast-like synoviocytes (FLS) exhibit an aggressive phenotype. Although the mechanisms responsible are not well defined, epigenetic determinants such as DNA methylation might contribute. DNA methyltransferases (DNMTs) are critical enzymes that
Vitalina M Komashko et al.
Epigenetics, 5(3), 229-240 (2010-03-23)
Methylation of DNA in combination with histone modifications establishes an epigenetic code that ensures the proper control of gene expression. Although DNA methyltransferases have been shown to interact with histone methyltransferases such as EZH2 (which methylates histone H3 on lysine
INSIG2 rs7566605 single nucleotide variant and global DNA methylation index levels are associated with weight loss in a personalized weight reduction program
Pirini F, et al.
Molecular Medicine Reports, 17(1), 1699-1709 (2018)
Rafael Guerrero-Preston et al.
Epigenetics, 5(6), 539-546 (2010-06-05)
Environmental exposures in-utero may alter the epigenome, thus impacting chromosomal stability and gene expression. We hypothesized that in utero exposures to maternal smoking and perfluoroalkyl compounds (PFCs) are associated with global DNA hypomethylation in umbilical cord serum. Our objective was
Magdalena Gryzinska et al.
Biochemical genetics, 51(7-8), 554-563 (2013-04-05)
DNA methylation is an epigenetic modification that plays an important role in the normal development and function of organisms. The level of DNA methylation is species-, tissue-, and organelle-specific, and the methylation pattern is determined during embryogenesis. DNA methylation has
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Articoli

Global DNA Methylation Quantification

The overall degree of methylation of a genome can be a useful measure of global regulatory changes. Measurement of this parameter is usually performed after complete digestion to the single base and then analyzed using HPLC or mass spectrophotometry. The Global DNA Modification Kit (MDQ1), allows the researcher to monitor DNA methylation using a format similar to a sandwich ELISA assay.

Protocolli

Imprint® Methylated DNA Quantification Kit (MDQ1) Protocol

Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

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