M9445

Matrix Metalloproteinase-2

recombinant, expressed in NSO cells, >95% (SDS-PAGE), buffered aqueous glycerol solution

• Sinonimo/i: 72 kD Gelatinase, 72 kD Type IV Collagenase, Gelatinase A, MMP-2
• Numero MDL: MFCD01324322
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Ricombinante: expressed in NSO cells
• Livello qualitativo: 200
• Saggio: >95% (SDS-PAGE)
• Forma fisica: buffered aqueous glycerol solution
• PM: apparent mol wt ~72 kDa
• N° accesso UniProt: P33434; P33436
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: mouse ... Mmp2(17390) ; rat ... Mmp2(81686)

Descrizione

Descrizione generale

Matrix Metalloproteinase-2 (MMP-2) also known as gelatinase or type IV collagenase is a 72kDa protein. MMP-2 is a member of matrix metalloproteinase (MMP) family of enzymes. Basic structure of MMP2 contains signal peptide domain that targets the enzyme for secretion, the pro-peptide domain, which is removed when the enzyme is activated and the catalytic site containing gelatin-binding domain.

Specificità

The amino acid sequences 1-662 of the proenzymes of MMP-2 are identical between mouse and rat.

Applicazioni

Matrix metalloproteinase-2 (MMP2) human has been used as a standard in zymography to measure proteolytic activity of MMP-2.

Azioni biochim/fisiol

Matrix Metalloproteinase-2 (MMP-2) cleaves gelatin, type IV, V, VII, X, and XI collagens, fibronectin, elastin, laminin, proteoglycans and a range of non extracellular matrix (ECM ) components. MMP-2 cleaves native type I collagen to N-terminal ¾ and C-terminal ¼ fragments identical to those generated by interstitial collagenases. MMP2 and MMP9 play an essential role in matrix degradation and they are implicated in the maintenance of neovascularization. In mice, deletion or inhibition of MMP2 protects against myocardial rupture.

MMP-2 degrades general matrix components and may have a role in processes such as host defense, cell proliferation, and protein turnover as well as tissue remodeling.

Stato fisico

Supplied as a 0.2 μm filtered solution of 25 mM Tris, pH 7.5, 5 mM calcium chloride, 75 mM sodium chloride, 0.025% Brij^® 35 and 50% glycerol.

Risultati analitici

The biological activity is measured by its ability to cleave a fluorogenic peptide sustrate.

Note legali

Brij is a registered trademark of Croda International PLC

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)