M8069
Monoclonal Anti-Mad1 antibody produced in mouse
~2 mg/mL, clone 9B10, purified immunoglobulin, buffered aqueous solution
Sinonimo/i:
Anti-Mitotic Arrest Deficient
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About This Item
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Coniugato
unconjugated
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
9B10, monoclonal
Stato
buffered aqueous solution
PM
antigen ~85 kDa
Reattività contro le specie
human
Concentrazione
~2 mg/mL
tecniche
immunocytochemistry: 10-20 μg/mL using HeLa cells
immunoprecipitation (IP): suitable
microarray: suitable
Isotipo
IgG2b
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... MAD1L1(8379)
Categorie correlate
Descrizione generale
Mitotic arrest deficient-like 1 (MAD1) is part of the cell-cycle checkpoint.
Monoclonal Anti-Mad1 (mouse IgG2b isotype) is derived from the hybridoma 9B10 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with human recombinant Mad1 protein.
Immunogeno
recombinant human Mad1 protein.
Applicazioni
Monoclonal Anti-Mad1 antibody produced in mouse has been used in
- immunoblotting
- immunoprecipitation
- immunocytochemistry
Azioni biochim/fisiol
Mitotic arrest deficient-like 1 (MAD1) functions as a regulator in the segregation of chromosomes during mitosis. It recruits mitotic arrest deficient-like 2 (MAD2) to kinetochores and directs it to function as an inhibitor. MAD1 also helps MAD2 to associate with translocated promoter region (TPr).
Stato fisico
Solution in 0.01 M phosphae buffered saline, pH 7.4, containing 15 mM sodium azide.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Efficient mitotic checkpoint signaling depends on integrated activities of Bub1 and the RZZ complex.
Gang Zhang et al.
The EMBO journal, 38(7) (2019-02-21)
Kinetochore localized Mad1 is essential for generating a "wait anaphase" signal during mitosis, hereby ensuring accurate chromosome segregation. Inconsistent models for the function and quantitative contribution of the two mammalian Mad1 kinetochore receptors: Bub1 and the Rod-Zw10-Zwilch (RZZ) complex exist.
Jun Wan et al.
Current biology : CB, 24(22), 2687-2692 (2014-12-03)
Mitotic arrest deficient 1 (Mad1) plays a well-characterized role in the major cell-cycle checkpoint that regulates chromosome segregation during mitosis, the mitotic checkpoint (also known as the spindle assembly checkpoint). During mitosis, Mad1 recruits Mad2 to unattached kinetochores, where Mad2
Garry G Sedgwick et al.
mAbs, 8(4), 689-697 (2016-03-18)
The spindle assembly checkpoint (SAC) ensures accurate chromosome segregation during mitosis by delaying the activation of the anaphase-promoting complex/cyclosome (APC/C) in response to unattached kinetochores. The Mad2 protein is essential for a functional checkpoint because it binds directly to Cdc20
Gang Zhang et al.
Journal of cell science, 125(Pt 13), 3243-3253 (2012-03-29)
The Ndc80 complex establishes end-on attachment of kinetochores to microtubules, which is essential for chromosome segregation. The Ndc80 subunit is characterized by an N-terminal region that binds directly to microtubules, and a long coiled-coil region that interacts with Nuf2. A
Timo E F Kuijt et al.
Chromosoma, 123(5), 471-480 (2014-04-04)
Fidelity of chromosome segregation is monitored by the spindle assembly checkpoint (SAC). Key components of the SAC include MAD1, MAD2, BUB1, BUB3, BUBR1, and MPS1. These proteins accumulate on kinetochores in early prometaphase but are displaced when chromosomes attach to
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