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Key Documents

L8292

Sigma-Aldrich

Lipoprotein, low density from human plasma

lyophilized powder

Sinonimo/i:

β-Lipoprotein, LDL, Low density lipoprotein

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About This Item

Numero CAS:
Numero MDL:
Codice UNSPSC:
12352202
NACRES:
NA.61

Origine biologica

human plasma

Livello qualitativo

Forma fisica

lyophilized powder

Confezionamento

vial of ~5 mg protein

tecniche

cell culture | stem cell: suitable

N° accesso UniProt

Temperatura di conservazione

2-8°C

Informazioni sul gene

human ... APOA1(335)

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Descrizione generale

Lipoprotein, low density (LDL) is synthesized by lipolysis and lipid transfer steps. They are formed from very low-density lipoprotein (VLDL) after uptake by LDL receptor in hepatocytes. LDL normal level of 130 mg/dL is desirable in human plasma. It is referred as bad cholesterol.

Applicazioni

Lipoprotein was used to study effect of the surface density of nano-segments immobilized on culture dishes on ex vivo expansion of hematopoietic stem and progenitor cells from umbilical cord blood. It was also used in the separation and cultivation of hematopoietic stem cells from umbilical cord blood by permeation through membranes with nano-segments.
Lipoprotein, low density from human plasma has been used:
  • in evaluating its anti-Langerhans cells (LC) differentiation activity in monocyte-derived Langerhans cells (MDLCs)
  • in the preparation of oxidized low-density lipoprotein (ox-LDL)
  • as a lipoprotein standard in quantifying embryonic cerebrospinal fluid (eCSF) from HH23 embryos by lipid staining

Azioni biochim/fisiol

LDL and HDL transport both dietary and endogenous cholesterol in the plasma. LDL is the main transporter of cholesterol and cholesteryl esters and makes up more than half of the total lipoprotein in plasma. LDL is absorbed by the liver and other tissues via receptor mediated endocytosis. The cytoplasmic domain of the LDL receptor facilitates the formation of coated pits; receptor-rich regions of the membrane. The ligand binding domain of the receptor recognizes apo-B100 on LDL, resulting in the formation of a clathrin-coated vesicle. ATP-dependent proton pumps lower the pH inside the vesicle resulting dissociation of LDL from its receptor. After loss of the clathrin coat the vesicles fuse with lysozomes, resulting in peptide and cholesteryl ester enzymatic hydrolysis. The LDL receptor can be recycled to the cell membrane. Insulin, tri-iodothyronine and dexamethasome have shown to be involved with the regulation of LDL receptor mediated uptake.
The apolipoprotein component of the LDL particle interacts with extracellular matrix in arterial intima resulting in their retention. LDL is the major driver of atherosclerotic cardiovascular disease progression.

Proprietà fisiche

Low density lipoproteins are smaller than VLDL and IDL (26 nm) (MW approximately 3.5 million) and more dense (~1.04). The protein component of LDL is apolipoprotein B100. LDL contains 20-22% protein, 10-15% triglycerides, 20-28% phospholipids, 37-48% cholesteryl esters and 8-10% cholesterol.

Nota sulla preparazione

Lyophilized from a solution of 0.15 M NaCl and 0.01% EDTA, pH 7.4

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


Certificati d'analisi (COA)

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LDL Cholesterol:" Bad" Cholesterol or Bad Science?
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Journal of American Physicians and Surgeons, 10(3), 83-83 (2005)
The formation of LDL: mechanisms and regulation.
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Journal of Lipid Research, 25(13), 1570-1576 (1984)
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Journal of immunology (Baltimore, Md. : 1950), 196(9), 3716-3728 (2016-03-27)
Langerhans cells (LCs) are epithelial APCs that sense danger signals and in turn trigger specific immune responses. In steady-state, they participate in the maintenance of peripheral tolerance to self-antigens whereas under inflammation LCs efficiently trigger immune responses in secondary lymphoid
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Umbilical cord blood (UCB) is an attractive source of hematopoietic stem and progenitor cells for hematopoietic stem cell (HSC) transplantation. However, the low number of HSCs obtainable from a single donor of UCB limits direct transplantation of UCB to the

Articoli

Since cholesterol is a water-insoluble molecule it must be packaged for transport within the plasma. The particles that package cholesterol, cholesteryl esters, and triglycerides for transport, are called lipoproteins.

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