I9640
IgG from bovine serum
technical grade, ≥80% (SDS-PAGE), buffered aqueous solution
Sinonimo/i:
Bovine IgG
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About This Item
Prodotti consigliati
Coniugato
unconjugated
Livello qualitativo
Grado
technical grade
Saggio
≥80% (SDS-PAGE)
Stato
buffered aqueous solution
tecniche
cell culture | mammalian: suitable
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
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Descrizione generale
Bovine immunoglobulin, protein source obtained from immunoglobulin derived from serum, is a naturally occurring bioactive proteins and peptides. Fetal calf or bovine serum IgG can be depleted with protein G affinity chromatography and various concentration of this product can be used in RPMI-1640 medium to produce mouse hybridoma cell line CAy-G (anti-HBs IgG1 mAb producing hybridoma cell). The heat aggregated bovine serum IgG is also useful in down regulating mannose receptor expression. IgG from bovine serum reacts specifically with mouse.
Immunogeno
IgG is isolated from pooled bovine serum.
Applicazioni
IgG from bovine serum is used as a supplement in cell culture.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.2 with 15 mM sodium azide as preservative
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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S Schreiber et al.
Journal of immunology (Baltimore, Md. : 1950), 147(4), 1377-1382 (1991-08-15)
We previously described the presence of an inhibitory protein contained in the 20 to 40% (NH4)2SO4 precipitable fraction of FCS that down-regulates expression of mannose receptors on bone marrow-derived macrophages. We now identify aggregated bovine IgG as the main inhibitory
C Aybay et al.
Journal of immunological methods, 233(1-2), 77-81 (2000-01-29)
Fetal calf serum (FCS) was depleted of its immunoglobulin G (IgG) in a rapid procedure using protein G affinity chromatography. 20 ml of FCS was depleted of its IgG in less than 80 min by applying 5 ml of FCS
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