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A8917

Sigma-Aldrich

Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sinonimo/i:

Rabbit Anti-Bovine IgG (whole molecule)−HRP

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About This Item

Numero MDL:
MFCD00162171
Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

rabbit

Coniugato

peroxidase conjugate

Forma dell’anticorpo

IgG fraction of antiserum

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

bovine

tecniche

direct ELISA: 1:20,000
dot blot: 1:80,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

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Descrizione generale

Immunoglobulin G (IgG) contributes to 10−20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.

Immunogeno

purified bovine IgG

Applicazioni

Anti-Bovine IgG (whole molecule) Peroxidase antibody produced in rabbit has been used in:
  • Dot- enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunohistology

Rabbit Anti-Bovine IgG (whole molecule)-Peroxidase antibody has been used for western blot and ELISA. The antibody can also be used for dot blot (1:80,000) and immunohistochemistry (1:1,000).

Azioni biochim/fisiol

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

Stato fisico

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Nota sulla preparazione

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H317,H334

Classi di pericolo

Resp. Sens. 1 - Skin Sens. 1

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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