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I2513

Sigma-Aldrich

Monoclonal Anti-Human IgG1 antibody produced in mouse

clone 8c/6-39, ascites fluid

Sinonimo/i:

Monoclonal Anti-Human IgG1 (Fc specific)

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

mouse

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

ascites fluid

Tipo di anticorpo

secondary antibodies

Clone

8c/6-39, monoclonal

contiene

15 mM sodium azide

tecniche

indirect ELISA: 1:400

Isotipo

IgG2a

Condizioni di spedizione

dry ice

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Descrizione generale

Monoclonal Anti-Human IgG1 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Human IgG consists of four subclasses (1-4) that can be recognized by antigen differences in their heavy chains. They constitute approximately 65, 25, 6 and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties.
The IgG subclass may be preferentially produced in response to different antigens. For instance, antipolysaccharide responses are mainly of the IgG2 subclass, while protein antigens give rise to IgG1 and IgG3 antibodies. Lipopolysaccahrides stimulate an IgG2 response in PBL′s and an IgG1 response in the spleen. Human IgG1 is the predominant subclass of in vivo and in vitro produced anti-tetanus toxoid antibodies. Only IgG1 and IgG3 are capable of adherence to mononuclear phagocytes. Serum IgG subclass deficiencies have been recorded for different patient groups. For example, IgG2 and IgG4 deficiency is associated with IgA deficiency as found in patients of ataxia telangiextasia. Low IgG2 levels were found in patients with SLE and juvenile diabetes melitus. A disproportionate elevation of IgG1 has also been found in the cerebral spinal fluid of patients with multiple sclerosis. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and may assist in the diagnosis of various disorders.
The antibody is specific for the Fc portion of human IgG1 and is non-reactive with other IgG subclasses. This clone, also described as HP-6091, was found to exhibit a valuable profile of reactivity and specificity for human IgG1 by the IUIS/WHO study.

Specificità

Human IgG consists of four subclasses (1-4) that can be recognized.

Applicazioni

Monoclonal Anti-Human IgG1 antibody produced in mouse has been used in:
  • direct hemagglutination(HA)
  • hemagglutination inhibition(HAI) assays
  • enzyme linked immunosorbent assay (ELISA)
  • immunofluorometric assay (IFMA)
  • immunofluorescence
  • cell-based assays for the detection of IgG subclasses of acetylcholine receptor (AChR) antibodies
Mouse monoclonal clone clone 8c/6-39 anti-Human IgG1 antibody may be used for the identification of human IgG1 subclass by means of various immunoassays and direct hemagglutination (HA) and hemagglutination inhibition (HAI) assays, enzyme linked immunosorbent assay (ELISA), immunofluorometric assay (IFMA) and detection of cytoplasmic IgG. The antibody may be useful in the analysis of the distribution of IgG subclasses in various diseases.

Azioni biochim/fisiol

Human IgG1 is the predominant subclass of in vivo and in vitro produced anti-tetanus toxoid antibodies. Only IgG1 and IgG3 are capable of adherence to mononuclear phagocytes via Fc receptors (FcR). A disproportionate elevation of IgG1 has also been found in the cerebral spinal fluid of patients with multiple sclerosis.

Stato fisico

The antibody is provided as ascites fluid with 0.1% sodium azide as a preservative.

Stoccaggio e stabilità

For continuous use, store at 0-5 °C. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Human IgG isotypes and activating Fcgamma receptors in the interaction of Salmonella enterica serovar Typhimurium with phagocytic cells
Goh YS, et al.
Immunology, 133(1), 74-74 (2011)
Pro-inflammatory pattern of IgG1 Fc glycosylation in multiple sclerosis cerebrospinal fluid
Wuhrer,M, et al.
Journal of Neuroinflammation, 12 (2015)
Chong Yan et al.
Journal of neuroimmunology, 349, 577403-577403 (2020-09-30)
Juvenile-onset myasthenia gravis (JOMG) is a unique clinical subtype in China, featured by a higher prevalence of ocular myasthenia gravis (OMG), higher seronegativity of acetylcholine receptor (AChR) antibodies, and better prognosis than that in adult-onset myasthenia gravis (AOMG). We previously
Determination of IgG Subclasses and Avidity of Antithyroid Peroxidase Antibodies in Patients with Subclinical Hypothyroidism A Comparison with Patients with Overt Hypothyroidism
Silva LM, et al.
Hormone Research in p?diatrics, 59(3), 118-124 (2003)
Chong Yan et al.
Pediatric neurology, 98, 74-79 (2019-07-17)
Patients in China with juvenile-onset myasthenia gravis present early, with a high prevalence of purely ocular symptoms, spontaneous remission rates, and low antibody seropositivity. Antibody detection using a cell-based assay has been reported to increase the diagnostic sensitivity in adult-onset

Articoli

Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.

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