H9408
β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei
lyophilized powder, ≥200 units/mg protein
Sinonimo/i:
(R)-3-Hydroxybutanoate:NAD+ oxidoreductase, 3-HBDH
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About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
Prodotti consigliati
Origine biologica
bacterial (Pseudomonas lemoignei)
Livello qualitativo
Stato
lyophilized powder
Attività specifica
≥200 units/mg protein
Purificato mediante
chromatography
Attività estranea
lactic dehydrogenase ≤0.05%
malic dehydrogenase ≤0.1%
Temperatura di conservazione
−20°C
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Descrizione generale
D-β-hydroxybutyrate dehydrogenase (BDH) is a membrane bound lipid-requiring enzyme.
Applicazioni
β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei has been used in coupled assay to confirm the formation of acetoacetate.
Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).
Azioni biochim/fisiol
In Paracoccus denitrificans, 3-hydroxybutyrate dehydrogenase plays a key role in the degradation of intracellular polyhydroxybutyrate and polyhydroxyvalerate.
In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
Definizione di unità
One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.
Stato fisico
lyophilized powder containing sucrose, β-NAD and Tris buffer salts
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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